Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 8ef9
Q-score: 0.605
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2023) 51 pp. 463-474 [ Pubmed: 36583344 DOI: doi:10.1093/nar/gkac1201 ]
- Dna (5'-d(*ap*gp*cp*ap*tp*cp*cp*gp*tp*ap*gp*(2da))-3') (5 kDa, DNA from Lates calcarifer)
- Ternary complex of lates calcarifer dna polymerase theta with duplex dna and incoming nucleotide (Complex from Lates calcarifer)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase theta (95 kDa, Protein from Lates calcarifer)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(*ap*gp*cp*tp*cp*tp*ap*cp*gp*gp*ap*tp*gp*c)-3') (8 kDa, DNA from Lates calcarifer)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8efc
Q-score: 0.492
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2023) 51 pp. 463-474 [ Pubmed: 36583344 DOI: doi:10.1093/nar/gkac1201 ]
- Ternary complex of lates calcarifer dna polymerase theta with duplex dna and incoming nucleotide (Complex from Lates calcarifer)
- Dna (5'-d(*ap*cp*tp*gp*tp*gp*ap*gp*gp*cp*ap*tp*cp*cp*gp*tp*ap*gp*(2da))-3') (6 kDa, DNA from Lates calcarifer)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dna polymerase theta (95 kDa, Protein from Lates calcarifer)
- Magnesium ion (24 Da, Ligand)
- Dna (5'-d(*ap*gp*cp*tp*cp*tp*ap*cp*gp*gp*ap*tp*gp*cp*cp*tp*cp*ap*cp*ap*g)-3') (8 kDa, DNA from Lates calcarifer)
Structure of Lates calcarifer DNA polymerase theta polymerase domain with hairpin DNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8efk
Q-score: 0.525
Li C, Zhu H, Jin S, Maksoud LM, Jain N, Sun J, Gao Y
Nucleic Acids Res (2023) 51 pp. 463-474 [ Pubmed: 36583344 DOI: doi:10.1093/nar/gkac1201 ]
- Lates calcarifer dna polymerase theta (95 kDa, Protein from Lates calcarifer)
- Magnesium ion (24 Da, Ligand)
- Ternary complex of lates calcarifer dna polymerase theta with duplex dna and incoming nucleotide (Complex from Lates calcarifer)
- 2',3'-dideoxyadenosine triphosphate (475 Da, Ligand)
- Dna (5'-d(p*tp*tp*tp*tp*gp*gp*cp*tp*tp*tp*tp*gp*cp*cp*(2da))-3') (7 kDa, DNA from Lates calcarifer)
CryoEM Structure of an Group II Intron Retroelement
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7uin
Q-score: 0.467
Chung K, Xu L, Chai P, Peng J, Devarkar SC, Pyle AM
Science (2022) 378 pp. 627-634 [ Pubmed: 36356138 DOI: doi:10.1126/science.abq2844 ]
- Magnesium ion (24 Da, Ligand)
- Ternary complex of rna, protein and dna (260 kDa, Complex from [Eubacterium] rectale)
- Water (18 Da, Ligand)
- Ammonium ion (18 Da, Ligand)
- Group ii intron reverse transcriptase/maturase (49 kDa, Protein from [Eubacterium] rectale)
- Dna (37-mer) (11 kDa, DNA from [Eubacterium] rectale)
- E.r iic intron (206 kDa, RNA from [Eubacterium] rectale)
Cryo-EM structure of Escherichia coli release complex of transcription termination (TTC-release)
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 7ypb
Q-score: 0.399
You L, Omollo EO, Yu C, Mooney RA, Shi J, Shen L, Wu X, Wen A, He D, Zeng Y, Feng Y, Landick R, Zhang Y
Nature (2023) 613 pp. 783-789 [ Pubmed: 36631609 DOI: doi:10.1038/s41586-022-05604-1 ]
- Rna (5'-r(*gp*cp*gp*up*cp*gp*cp*ap*gp*gp*cp*cp*up*up*up*up*up*ap*up*u)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase (Complex)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna (31-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna/rna (Complex)
- E. coli release complex of transcription termination (400 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(*gp*gp*cp*cp*up*gp*cp*gp*ap*cp*gp*a)-3') (3 kDa, RNA from synthetic construct)
Cryo-EM structure of E.coli retron-Ec86 (RT-msDNA-RNA) at 3.2 angstrom
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 7v9u
Q-score: 0.471
Wang Y, Guan Z, Wang C, Nie Y, Chen Y, Qian Z, Cui Y, Xu H, Wang Q, Zhao F, Zhang D, Tao P, Sun M, Yin P, Jin S, Wu S, Zou T
Nat Microbiol (2022) 7 pp. 1480-1489 [ Pubmed: 35982312 DOI: doi:10.1038/s41564-022-01197-7 ]
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (36 kDa, Protein from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna (5'-r(p*cp*gp*up*ap*ap*gp*gp*g)-3') (4 kDa, RNA from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
Cryo-EM structure of E.coli retron-Ec86 in complex with its effector at 2.8 angstrom
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7v9x
Q-score: 0.549
To Be Published
- Retron st85 family effector protein (35 kDa, Protein from Escherichia coli)
- Dna (105-mer) (32 kDa, DNA from Escherichia coli)
- Retron-ec86 (rt-msdna-rna) (Complex from Escherichia coli)
- Rna (14-mer) (4 kDa, RNA from Escherichia coli)
- Rna (81-mer) (25 kDa, RNA from Escherichia coli)
- Rna-directed dna polymerase from retron ec86 (37 kDa, Protein from Escherichia coli)
Tetrahymena CST with Polymerase alpha-Primase
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7uy7
Q-score: 0.293
He Y, Song H, Chan H, Liu B, Wang Y, Susac L, Zhou ZH, Feigon J
Nature (2022) 608 pp. 813-818 [ DOI: doi:10.1038/s41586-022-04931-7 Pubmed: 35831498 ]
- Telomerase associated protein p50 (50 kDa, Protein from Tetrahymena thermophila)
- Tetrahymena cst (Complex from Tetrahymena thermophila)
- Telomere dna (19 kDa, DNA from Tetrahymena thermophila)
- Tetrahymena cst-polaprim (Complex)
- Telomerase-associated protein of 45 kda (43 kDa, Protein from Tetrahymena thermophila)
- Zinc ion (65 Da, Ligand)
- Dna polymerase (161 kDa, Protein from Tetrahymena thermophila)
- Telomerase-associated protein of 75 kda (73 kDa, Protein from Tetrahymena thermophila)
- Tetrahymena dna polymerase alpha-primase (Complex from Tetrahymena thermophila)
- Telomerase-associated protein of 19 kda (19 kDa, Protein from Tetrahymena thermophila)
Cryo-EM structure of human CST bound to DNA polymerase alpha-primase in a recruitment state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7u5c
Q-score: 0.33
Cai SW, Zinder JC, Svetlov V, Bush MW, Nudler E, Walz T, de Lange T
Nat Struct Mol Biol (2022) 29 pp. 813-819 [ Pubmed: 35578024 DOI: doi:10.1038/s41594-022-00766-y ]
- Dna primase small subunit (49 kDa, Protein from Homo sapiens)
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha catalytic subunit (129 kDa, Protein from Homo sapiens)
- Cst complex subunit stn1 (42 kDa, Protein from Homo sapiens)
- Cst complex subunit ten1 (13 kDa, Protein from Homo sapiens)
- Dna polymerase alpha subunit b (66 kDa, Protein from Homo sapiens)
- Human cst bound to dna polymerase alpha(pola1 delta n)-primase in a recruitment state (500 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Cst complex subunit ctc1 (135 kDa, Protein from Homo sapiens)
- Dna primase large subunit (59 kDa, Protein from Homo sapiens)
- Canonical telomeric dna sequence (5 kDa, DNA from Homo sapiens)
Human telomerase catalytic core structure at 3.3 Angstrom
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7trd
Q-score: 0.447
Liu B, He Y, Wang Y, Song H, Zhou ZH, Feigon J
Nature (2022) 604 pp. 578-583 [ Pubmed: 35418675 DOI: doi:10.1038/s41586-022-04582-8 ]
- Telomeric repeat substrate (5 kDa, DNA from Homo sapiens)
- Telomerase rna, partial sequence (145 kDa, RNA from Homo sapiens)
- Telomerase reverse transcriptase (130 kDa, Protein from Homo sapiens)
- Human telomerase catalytic core with dna-substrate (Complex from Homo sapiens)