Vanadate-trapped BSEP in nanodiscs
Resolution: 2.81 Å
EM Method: Single-particle
Fitted PDBs: 8pmj
Q-score: 0.59
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ DOI: doi:10.1038/s41467-023-43109-1 Pubmed: 37949847 ]
- Vanadate-trapped bsep in nanodiscs (146 kDa, Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Vanadate ion (114 Da, Ligand)
Human bile salt export pump (BSEP) in complex with inhibitor GBM in nanodiscs
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8pm6
Q-score: 0.428
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ Pubmed: 37949847 DOI: doi:10.1038/s41467-023-43109-1 ]
- 5-chloro-n-(2-{4-[(cyclohexylcarbamoyl)sulfamoyl]phenyl}ethyl)-2-methoxybenzamide (494 Da, Ligand)
- The complex of bsep with inhibitor gbm (146 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
Nucleotide-bound BSEP in nanodiscs
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8pmd
Q-score: 0.519
Liu H, Irobalieva RN, Kowal J, Ni D, Nosol K, Bang-Sorensen R, Lancien L, Stahlberg H, Stieger B, Locher KP
Nat Commun (2023) 14 pp. 7296-7296 [ Pubmed: 37949847 DOI: doi:10.1038/s41467-023-43109-1 ]
- Nucleotide-bound bsep in nanodiscs (146 kDa, Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Bile salt export pump (146 kDa, Protein from Homo sapiens)
Structure of 5D3-Fab and nanobody(Nb17)-bound ABCG2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8p8a
Q-score: 0.52
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol (2023) 435 pp. 168234-168234 [ DOI: doi:10.1016/j.jmb.2023.168234 Pubmed: 37597690 ]
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Structure of nanobody (nb8)-bound abcg2 (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) heavy chain variable domain (17 kDa, Protein from Mus musculus)
- Nanobody (13 kDa, Protein from Lama glama)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7oj8
Q-score: 0.437
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- Estrone 3-sulfate (350 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
- Abcg2 in complex with e1s under turnover condition (144 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
ABCG2 topotecan turnover-1 state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ojh
Q-score: 0.513
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione (421 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Abcg2 in complex with topotecan under turnover condition (144 kDa, Complex from Homo sapiens)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
ABCG2 topotecan turnover-2 state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7oji
Q-score: 0.485
Yu Q, Ni D, Kowal J, Manolaridis I, Jackson SM, Stahlberg H, Locher KP
Nat Commun (2021) 12 pp. 4376-4376 [ DOI: doi:10.1038/s41467-021-24651-2 Pubmed: 34282134 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Abcg2 in complex with topotecan under turnover condition (144 kDa, Complex from Homo sapiens)
- Broad substrate specificity atp-binding cassette transporter abcg2 (73 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (s)-10-[(dimethylamino)methyl]-4-ethyl-4,9-dihydroxy-1h-pyrano[3',4':6,7]inolizino[1,2-b]-quinoline-3,14(4h,12h)-dione (421 Da, Ligand)