Cryo-EM structure of human NTCP-Bulevirtide complex
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rqf
Q-score: 0.492
Liu H, Zakrzewicz D, Nosol K, Irobalieva RN, Mukherjee S, Bang-Sorensen R, Goldmann N, Kunz S, Rossi L, Kossiakoff AA, Urban S, Glebe D, Geyer J, Locher KP
To Be Published
- Polymerase (5 kDa, Protein from hepatitis B virus genotype C)
- Light chain of fab3 (23 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- N-tetradecanoylglycine (285 Da, Ligand)
- Heavy chain of fab3 (25 kDa, Protein from Homo sapiens)
- Structure of bulevirtide-bound human ntcp in complex with fab and nanobody (110 kDa, Complex from Homo sapiens)
- Fab-specific nanobody (13 kDa, Protein from Lama glama)
Structure of 5D3-Fab and nanobody(Nb17)-bound ABCG2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8p8a
Q-score: 0.52
Irobalieva RN, Manolaridis I, Jackson SM, Ni D, Pardon E, Stahlberg H, Steyaert J, Locher KP
J Mol Biol (2023) 435 pp. 168234-168234 [ DOI: doi:10.1016/j.jmb.2023.168234 Pubmed: 37597690 ]
- 5d3(fab) light chain variable domain (23 kDa, Protein from Mus musculus)
- Structure of nanobody (nb8)-bound abcg2 (Complex from Homo sapiens)
- Atp-binding cassette sub-family g member 2 (72 kDa, Protein from Homo sapiens)
- 5d3(fab) heavy chain variable domain (17 kDa, Protein from Mus musculus)
- Nanobody (13 kDa, Protein from Lama glama)
Structure of the human sodium/bile acid cotransporter (NTCP) in complex with Fab and nanobody
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7zyi
Q-score: 0.502
Liu H, Irobalieva RN, Bang-Sorensen R, Nosol K, Mukherjee S, Agrawal P, Stieger B, Kossiakoff AA, Locher KP
Cell Res (2022) 32 pp. 773-776 [ Pubmed: 35726088 DOI: doi:10.1038/s41422-022-00680-4 ]
- Nanobody (13 kDa, Protein from Lama glama)
- Sodium/bile acid cotransporter (ntcp) in complex with fab and nanobody (110 kDa, Complex from Homo sapiens)
- Heavy chain of fab (25 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Sodium/bile acid cotransporter (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Light chain of fab (23 kDa, Protein from Mus musculus)
- Glycochenodeoxycholic acid (449 Da, Ligand)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7php
Q-score: 0.505
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Multidrug resistance protein norm (52 kDa, Protein from Vibrio cholerae RC385)
- Norm-nb17_4 (14 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
Resolution: 8.45 Å
EM Method: Single-particle
Fitted PDBs: 7phq
Q-score: 0.111
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Dmt-nb16_4 (14 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7pij
Q-score: 0.422
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Dmtnb16_4 (14 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)