Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
To Be Published
- Amino group (16 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
To Be Published
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
Human Cholecystokinin 1 receptor (CCK1R) Gs complex
Resolution: 1.95 Å
EM Method: Single-particle
Fitted PDBs: 7mbx
Q-score: 0.619
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ DOI: doi:10.1371/journal.pbio.3001295 Pubmed: 34086670 ]
- Cck1r/cck-8/gas complex (140 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Cholecystokinin-8 (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (48 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunits alpha and gamma (Complex from Homo sapiens)
- Cholecystokinin receptor type a (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Rattus norvegicus)
Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7mby
Q-score: 0.413
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ Pubmed: 34086670 DOI: doi:10.1371/journal.pbio.3001295 ]
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
- Cck-8 (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (29 kDa, Protein from Homo sapiens)
- G protein subunit beta-1 (Complex from Rattus norvegicus)
- Cck1r (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G(sqi)-alpha/g protein subunit gamma-2 (Complex from Homo sapiens)
- Cck1r/cck-8/mgsqi complex (150 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Human secretin receptor Gs complex
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6wi9
Q-score: 0.359
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ Pubmed: 32811827 DOI: doi:10.1038/s41467-020-17791-4 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Gs coupled sectretin complex with secretin peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Secretin (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Secretin (3 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
Human secretin receptor Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 6wzg
Q-score: 0.609
Dong M, Deganutti G, Piper SJ, Liang YL, Khoshouei M, Belousoff MJ, Harikumar KG, Reynolds CA, Glukhova A, Furness SGB, Christopoulos A, Danev R, Wootten D, Sexton PM, Miller LJ
Nat Commun (2020) 11 pp. 4137-4137 [ DOI: doi:10.1038/s41467-020-17791-4 Pubmed: 32811827 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, secretin receptor (Complex from Homo sapiens)
- Gs coupled secretin complex with secretin peptide (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Secretin (Complex from Homo sapiens)
- Nanobody35 (Complex from Lama glama)
- Secretin receptor (51 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Secretin (3 kDa, Protein from Homo sapiens)