Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ DOI: doi:10.1038/s41467-023-41199-5 Pubmed: 37673901 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- Xanomeline (281 Da, Ligand)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
Human Cholecystokinin 1 receptor (CCK1R) Gs complex
Resolution: 1.95 Å
EM Method: Single-particle
Fitted PDBs: 7mbx
Q-score: 0.619
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ DOI: doi:10.1371/journal.pbio.3001295 Pubmed: 34086670 ]
- Cck1r/cck-8/gas complex (140 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Cholecystokinin-8 (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (48 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(s) subunits alpha and gamma (Complex from Homo sapiens)
- Cholecystokinin receptor type a (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Rattus norvegicus)
Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7mby
Q-score: 0.413
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ Pubmed: 34086670 DOI: doi:10.1371/journal.pbio.3001295 ]
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
- Cck-8 (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (29 kDa, Protein from Homo sapiens)
- G protein subunit beta-1 (Complex from Rattus norvegicus)
- Cck1r (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G(sqi)-alpha/g protein subunit gamma-2 (Complex from Homo sapiens)
- Cck1r/cck-8/mgsqi complex (150 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)