Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
To Be Published
- Amino group (16 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
To Be Published
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-PACAP27-Gs complex
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8e3y
Q-score: 0.576
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Pituitary adenylate cyclase activating polypeptide-27 (Complex from Homo sapiens)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with pacap27 peptide (Complex)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Pituitary adenylate cyclase-activating polypeptide (3 kDa, Protein from Homo sapiens)
Cryo-EM structure of the VPAC1R-VIP-Gs complex
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8e3z
Q-score: 0.483
Piper SJ, Deganutti G, Lu J, Zhao P, Liang YL, Lu Y, Fletcher MM, Hossain MA, Christopoulos A, Reynolds CA, Danev R, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 7013-7013 [ DOI: doi:10.1038/s41467-022-34629-3 Pubmed: 36385145 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short, guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1, guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2, vasoactive intestinal polypeptide receptor 1 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Vasoactive intestinal peptide (3 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Gs coupled vasoactive intestinal polypeptide receptor 1 (vpac1) complex with vip peptide (Complex)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Vasoactive intestinal peptide-vip (Complex from Homo sapiens)
- Vasoactive intestinal polypeptide receptor 1 (52 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
Exendin-4-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7lll
Q-score: 0.479
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ Pubmed: 35013280 DOI: doi:10.1038/s41467-021-27760-0 ]
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Exendin-4 (4 kDa, Protein from Heloderma suspectum)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Exendin-4-glp-1r-gs complex (Complex from Homo sapiens)
Oxyntomodulin-bound Glucagon-Like Peptide-1 (GLP-1) Receptor in complex with Gs protein
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lly
Q-score: 0.452
Deganutti G, Liang YL, Zhang X, Khoshouei M, Clydesdale L, Belousoff MJ, Venugopal H, Truong TT, Glukhova A, Keller AN, Gregory KJ, Leach K, Christopoulos A, Danev R, Reynolds CA, Zhao P, Sexton PM, Wootten D
Nat Commun (2022) 13 pp. 92-92 [ DOI: doi:10.1038/s41467-021-27760-0 Pubmed: 35013280 ]
- Pro-glucagon (4 kDa, Protein from Sus scrofa)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Glucagon-like peptide 1 receptor (56 kDa, Protein from Homo sapiens)
- Oxyntomodulin-glp-1r-gs complex (Complex from Homo sapiens)
- Nb35 (13 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)