cryo-EM structure of the human respiratory complex II
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8gs8
Q-score: 0.577
Du Z, Zhou X, Lai Y, Xu J, Zhang Y, Zhou S, Feng Z, Yu L, Tang Y, Wang W, Yu L, Tian C, Ran T, Chen H, Guddat LW, Liu F, Gao Y, Rao Z, Gong H
PNAS (2023) 120 pp. e2216713120-e2216713120 [ DOI: doi:10.1073/pnas.2216713120 Pubmed: 37098072 ]
- Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial (17 kDa, Protein from Homo sapiens)
- Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial (31 kDa, Protein from Homo sapiens)
- Succinate dehydrogenase cytochrome b560 subunit, mitochondrial (18 kDa, Protein from Homo sapiens)
- Fe3-s4 cluster (295 Da, Ligand)
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (72 kDa, Protein from Homo sapiens)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- The human mitochondrial complex ii, composed of sdha, sdhb, sdhc and sdhd (Complex from Homo sapiens)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Ubiquinone-1 (250 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
Mycobacterium smegmatis Sdh1 in complex with UQ1
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 7d6v
Q-score: 0.636
Zhou X, Gao Y, Wang W, Yang X, Yang X, Liu F, Tang Y, Lam SM, Shui G, Yu L, Tian C, Guddat LW, Wang Q, Rao Z, Gong H
PNAS (2021) 118 [ Pubmed: 33876763 DOI: doi:10.1073/pnas.2022308118 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fumarate reductase iron-sulfur subunit (28 kDa, Protein from Mycolicibacterium smegmatis)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Succinate dehydrogenase subunit a (70 kDa, Protein from Mycolicibacterium smegmatis)
- Mycobacterium smegmatis sdh1 in complex with uq1 (Complex from Mycolicibacterium smegmatis MC2 51)
- Ubiquinone-1 (250 Da, Ligand)
- Succinate dehydrogenase (membrane anchor subunit) (32 kDa, Protein from Mycolicibacterium smegmatis)
Mycobacterium smegmatis Sdh1 complex in the apo form
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 7d6x
Q-score: 0.587
Zhou X, Gao Y, Wang W, Yang X, Yang X, Liu F, Tang Y, Lam SM, Shui G, Yu L, Tian C, Guddat LW, Wang Q, Rao Z, Gong H
PNAS (2021) 118 [ Pubmed: 33876763 DOI: doi:10.1073/pnas.2022308118 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Fe3-s4 cluster (295 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fumarate reductase iron-sulfur subunit (28 kDa, Protein from Mycolicibacterium smegmatis)
- Mycobacterium smegmatis sdh1 complex in apo form (Complex from Mycolicibacterium smegmatis MC2 51)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Succinate dehydrogenase subunit a (70 kDa, Protein from Mycolicibacterium smegmatis)
- Succinate dehydrogenase (membrane anchor subunit) (32 kDa, Protein from Mycolicibacterium smegmatis)
Mycobacterium smegmatis SugABC complex
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7cad
Q-score: 0.505
Liu F, Liang J, Zhang B, Gao Y, Yang X, Hu T, Yang H, Xu W, Guddat LW, Rao Z
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abb9833 Pubmed: 33127676 ]
- Abc transporter, atp-binding protein sugc (43 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Mycobacterium smegmatis sugabc complex (Complex from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, permease protein sugb (29 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Abc sugar transporter, permease component (32 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis LpqY-SugABC complex in the resting state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 7cae
Q-score: 0.491
Liu F, Liang J, Zhang B, Gao Y, Yang X, Hu T, Yang H, Xu W, Guddat LW, Rao Z
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abb9833 Pubmed: 33127676 ]
- Abc transporter, permease protein sugb (29 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Bacterial extracellular solute-binding protein (50 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Mycobacterium smegmatis lpqy-sugabc complex in the resting state. (Complex from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, atp-binding protein sugc (43 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Abc sugar transporter, permease component (32 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
Mycobacterium smegmatis LpqY-SugABC complex in the pre-translocation state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7caf
Q-score: 0.498
Liu F, Liang J, Zhang B, Gao Y, Yang X, Hu T, Yang H, Xu W, Guddat LW, Rao Z
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abb9833 Pubmed: 33127676 ]
- Abc transporter, atp-binding protein sugc (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Abc transporter, permease protein sugb (29 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Mycobacterium smegmatis lpqy-sugabc complex in the pre-translocation state (Complex from Mycolicibacterium smegmatis MC2 155)
- Abc sugar transporter, permease component (32 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
- Bacterial extracellular solute-binding protein (50 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mycobacterium smegmatis LpqY-SugABC complex in the catalytic intermediate state
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7cag
Q-score: 0.453
Liu F, Liang J, Zhang B, Gao Y, Yang X, Hu T, Yang H, Xu W, Guddat LW, Rao Z
Sci Adv (2020) 6 [ DOI: doi:10.1126/sciadv.abb9833 Pubmed: 33127676 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Abc transporter, permease protein sugb (29 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Mycobacterium smegmatis lpqy-sugabc complex in the catalytic intermediate state (Complex from Mycolicibacterium smegmatis MC2 155)
- Bacterial extracellular solute-binding protein (50 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Magnesium ion (24 Da, Ligand)
- Abc transporter, atp-binding protein sugc (43 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))
- Abc sugar transporter, permease component (32 kDa, Protein from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155))