Structure of the mu opioid receptor bound to the antagonist nanobody NbE
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qot
Q-score: 0.413
Yu J, Kumar A, Zhang X, Martin C, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
bioRxiv (2023) [ Pubmed: 38106026 DOI: doi:10.1101/2023.12.06.570395 ]
- Nabfab hc (28 kDa, Protein from synthetic construct)
- Nabfab lc (25 kDa, Protein from synthetic construct)
- Mu-type opioid receptor (53 kDa, Protein from Mus musculus)
- Nanobody e (nbe) (18 kDa, Protein from Lama glama)
- Anti-fab nanobody (17 kDa, Protein from Lama glama)
- Mor-nbe-nabfab-antifab complex (120 kDa, Complex from Mus musculus)
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 8pm2
Q-score: 0.496
Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Verprintsev DB, Vaidehi N, Weyand SN, Tate CG
bioRxiv (2023) [ Pubmed: 37461561 DOI: doi:10.1101/2023.07.07.547762 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- ~{n},~{n}-dimethylcyclohexanamine (127 Da, Ligand)
- A complex of mouse trace-amine associated receptor 7f solubilized in lmng/chs bound to n,n-dimethylcyclohexylamine and coupled to: engineered guanine nucleotide-binding protein g(s) subunit alpha isoform short + guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 + guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 + nanobody 35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Nanobody 35 (16 kDa, Protein from Lama glama)
- Trace amine-associated receptor 7f (41 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7mby
Q-score: 0.413
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ Pubmed: 34086670 DOI: doi:10.1371/journal.pbio.3001295 ]
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Scfv16 (Complex from Mus musculus)
- Cck-8 (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (29 kDa, Protein from Homo sapiens)
- G protein subunit beta-1 (Complex from Rattus norvegicus)
- Cck1r (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- G(sqi)-alpha/g protein subunit gamma-2 (Complex from Homo sapiens)
- Cck1r/cck-8/mgsqi complex (150 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)