Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class1)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6k7g
Q-score: 0.494
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1 state class2)
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 6k7h
Q-score: 0.487
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class2)
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 6k7i
Q-score: 0.494
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ATP state class1)
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 6k7j
Q-score: 0.526
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphomethylphosphonic acid adenylate ester (505 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1-ADP-Pi state)
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6k7k
Q-score: 0.538
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2P state class2)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 6k7l
Q-score: 0.552
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E2Pi-PL state)
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 6k7m
Q-score: 0.551
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- (2~{s})-2-azanyl-3-[[(2~{r})-3-hexadecanoyloxy-2-[(~{z})-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-propanoic acid (762 Da, Ligand)
Cryo-EM structure of the human P4-type flippase ATP8A1-CDC50 (E1P state)
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 6k7n
Q-score: 0.511
Hiraizumi M, Yamashita K, Nishizawa T, Nureki O
Science (2019) 365 pp. 1149-1155 [ DOI: doi:10.1126/science.aay3353 Pubmed: 31416931 ]
- Tetrafluoroaluminate ion (102 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cell cycle control protein 50a (40 kDa, Protein from Homo sapiens)
- Phospholipid-transporting atpase (130 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Atp8a1-cdc50a (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)