Electron Microscopy Data Bank

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initial_angle_assignment_type:"MAXIMUM LIKELIHOOD" AND xref_links:"pdb" AND assembly_molecular_weight:{2000000 TO 5000000] AND author_orcid:"0000-0001-6546-2597" AND author_name:"Gamper HB"

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EMD-25405

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Late translocation intermediate with EF-G partially dissociated (Structure V)

Release date: Feb. 23, 2022
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7ss9
Q-score: 0.308

Carbone CE, Loveland AB, Gamper Jr HB, Hou YM, Demo G, Korostelev AA

Nat Commun (2021) 12 pp. 7236-7236 [ DOI: doi:10.1038/s41467-021-27415-0 Pubmed: 34903725 ]

Sample:

30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
Elongation factor g (76 kDa, Protein from Escherichia coli 113303)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
Mrna (12 kDa, RNA from Escherichia coli K-12)
Trna pro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
5s rrna (38 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
Trna fmet (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
16s rrna (498 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
Late translocation intermediate with ef-g partially dissociated (structure v) (2 MDa, Complex from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l1 (24 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
23s rrna (941 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-22669

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Cryo-EM map of pre-translocation non-frameshifting(CCA-A) complex (Structure I)

Release date: July 28, 2021
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7k50
Q-score: 0.407

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli K-12)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
Pre-translocation non-frameshifting(cca-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
Trnafmet (24 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
16s ribosomal rna (498 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
Mrna (5 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
Trnapro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-22670

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Cryo-EM map of mid-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure II)

Release date: July 28, 2021
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7k51
Q-score: 0.369

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
Mrna (5 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
Trnafmet (24 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli K-12)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
Elongation factor g (78 kDa, Protein from Escherichia coli K-12)
Proline (115 Da, Ligand)
16s ribosomal rna (498 kDa, RNA from Escherichia coli K-12)
5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
Trnapro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
Mid-translocated non-frameshifting(cca-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-22671

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Cryo-EM map of near post-translocated non-frameshifting(CCA-A) complex with EF-G and GDPCP (Structure III)

Release date: July 28, 2021
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7k52
Q-score: 0.375

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
Mrna (5 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
Trnafmet (24 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli K-12)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
Elongation factor g (78 kDa, Protein from Escherichia coli K-12)
Proline (115 Da, Ligand)
16s ribosomal rna (498 kDa, RNA from Escherichia coli K-12)
5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
Trnapro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
Near post-translocated non-frameshifting(cca-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)

[Show more]

EMD-22672

Download Map 3D View

Cryo-EM map of pre-translocation +1-frameshifting(CCC-A) complex (Structure I-FS)

Release date: July 28, 2021
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7k53
Q-score: 0.437

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
16s ribosomal rna (498 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Trnafmet (24 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
Mrna (5 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli (strain K12))
5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
Trnapro (24 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
Pre-translocation +1-frameshifting(ccc-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
23s ribosomal rna (941 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
N-formylmethionine (177 Da, Ligand)

[Show more]

EMD-22673

Download Map 3D View

Cryo-EM of mid-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure II-FS)

Release date: July 28, 2021
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7k54
Q-score: 0.406

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli (strain K12))
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
Trnafmet (24 kDa, RNA from Escherichia coli)
Proline (115 Da, Ligand)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
Mid-translocated +1-frameshifting(ccc-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
Elongation factor g (78 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
Trnapro (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
N-formylmethionine (177 Da, Ligand)
Mrna (5 kDa, RNA from Escherichia coli K-12)

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EMD-22674

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Cryo-EM map of near post-translocated +1-frameshifting(CCC-A) complex with EF-G and GDPCP (Structure III-FS)

Release date: July 28, 2021
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7k55
Q-score: 0.394

Demo G, Gamper HB, Loveland AB, Masuda I, Carbone CE, Svidritskiy E, Hou YM, Korostelev AA

Nat Commun (2021) 12 pp. 4644-4644 [ Pubmed: 34330903 DOI: doi:10.1038/s41467-021-24911-1 ]

Sample:

50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
16s ribosomal rna (498 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
Trnafmet (24 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli (strain K12))
5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
Proline (115 Da, Ligand)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
Near post-translocated +1-frameshifting(ccc-a) ribosome complex (1 MDa, Complex from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli (strain K12))
Trnapro (24 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
Elongation factor g (78 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l10 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
23s ribosomal rna (941 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
N-formylmethionine (177 Da, Ligand)
Mrna (5 kDa, RNA from Escherichia coli K-12)

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