Electron Microscopy Data Bank

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Structure of the yeast Gcn1 bound to a colliding stalled 80S ribosome with MBF1, A/P-tRNA and P/E-tRNA

Release date: April 14, 2021
Resolution: 4.36 Å
EM Method: Single-particle
Fitted PDBs: 7nrd
Q-score: 0.363

Pochopien AA, Beckert B, Kasvandik S, Berninghausen O, Beckmann R, Tenson T, Wilson DN

PNAS (2021) 118 [ Pubmed: 33790014 DOI: doi:10.1073/pnas.2022756118 ]

Sample:

60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l34-a (12 kDa, Protein from Baker's yeast)
Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Baker's yeast)
40s ribosomal protein s15 (13 kDa, Protein from Baker's yeast)
40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s13 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s3 (24 kDa, Protein from Baker's yeast)
Multiprotein-bridging factor 1 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l6-b (18 kDa, Protein from Baker's yeast)
40s ribosomal protein s0-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
40s ribosomal protein s11-a (16 kDa, Protein from Baker's yeast)
60s ribosomal protein l41-b (3 kDa, Protein from Baker's yeast)
60s ribosomal protein l8-a (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s6-a (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s5 (22 kDa, Protein from Baker's yeast)
40s ribosomal protein s26-b (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s10-a (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
60s ribosomal protein l42-a (11 kDa, Protein from Baker's yeast)
40s ribosomal protein s8-a (21 kDa, Protein from Baker's yeast)
40s ribosomal protein s12 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l24-a (7 kDa, Protein from Baker's yeast)
60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s14-b (13 kDa, Protein from Baker's yeast)
Trna (77-mer) (24 kDa, RNA from Saccharomyces cerevisiae S288c)
60s ribosomal protein l25 (13 kDa, Protein from Baker's yeast)
60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
40s ribosomal protein s9-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)
60s ribosomal protein l22-a (11 kDa, Protein from Baker's yeast)
40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s25-a (7 kDa, Protein from Baker's yeast)
60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s28-a (7 kDa, Protein from Baker's yeast)
40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
Tpa_inf: saccharomyces cerevisiae s288c chromosome xii, complete sequence (579 kDa, RNA from Saccharomyces cerevisiae S288C)
60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
40s ribosomal protein s18-a (16 kDa, Protein from Baker's yeast)
60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
5s rrna (121-mer) (38 kDa, RNA from Saccharomyces cerevisiae S288c)
60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
40s ribosomal protein s23-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
40s ribosomal protein s30-a (5 kDa, Protein from Baker's yeast)
60s ribosomal protein l43-a (9 kDa, Protein from Baker's yeast)
Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Baker's yeast)
60s ribosomal protein l11-b (19 kDa, Protein from Baker's yeast)
Structure of the yeast gcn1-bound coilliding stalled 80s ribosome with mbf1, gir2, a/p-trna and p/e-trna (Complex from Saccharomyces cerevisiae S288c)
60s ribosomal protein l14-a (14 kDa, Protein from Baker's yeast)
25s rrna (3184-mer) (1 MDa, RNA from Saccharomyces cerevisiae S288C)
40s ribosomal protein s20 (11 kDa, Protein from Baker's yeast)
60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
5.8s rrna (158-mer) (50 kDa, RNA from Saccharomyces cerevisiae S288c)
60s ribosomal protein l7-a (25 kDa, Protein from Baker's yeast)
40s ribosomal protein s2 (23 kDa, Protein from Baker's yeast)
60s ribosomal protein l13-a (21 kDa, Protein from Baker's yeast)
60s ribosomal protein l30 (10 kDa, Protein from Baker's yeast)
60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
40s ribosomal protein s1-a (24 kDa, Protein from Baker's yeast)
40s ribosomal protein s17-b (14 kDa, Protein from Baker's yeast)
60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
Trna (75-mer) (24 kDa, RNA from Baker's yeast)
60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
Mrna (32-mer) (9 kDa, RNA from Saccharomyces cerevisiae S288c)

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