Cryo-EM structure of ALC1/CHD1L bound to a PARylated nucleosome
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7otq
Q-score: 0.282
Bacic L, Gaullier G, Sabantsev A, Lehmann LC, Brackmann K, Dimakou D, Halic M, Hewitt G, Boulton S, Deindl S, Workman JL
eLife (2021) 10 [ DOI: doi:10.7554/eLife.71420 Pubmed: 34486521 ]
- Histone h3.2 (15 kDa, Protein from Xenopus laevis)
- Histone h2a type 1 (14 kDa, Protein from Xenopus laevis)
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
- Dna (149-mer) widom 601 sequence (49 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Chromodomain-helicase-dna-binding protein 1-like (98 kDa, Protein from Homo sapiens)
- Alc1/chd1l bound to a parylated nucleosome (305 kDa, Complex from Xenopus laevis)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
Mfd-bound E.coli RNA polymerase elongation complex - II state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6x43
Q-score: 0.476
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ Pubmed: 33480355 DOI: doi:10.7554/eLife.62117 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Escherichia coli mfd-rna polymerase elongation complex: state ii (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - III state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6x4w
Q-score: 0.375
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ Pubmed: 33480355 DOI: doi:10.7554/eLife.62117 ]
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state iii (570 kDa, Complex from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
Mfd-bound E.coli RNA polymerase elongation complex - IV state
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6x4y
Q-score: 0.443
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ Pubmed: 33480355 DOI: doi:10.7554/eLife.62117 ]
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state iv (570 kDa, Complex from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
Mfd-bound E.coli RNA polymerase elongation complex - V state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6x50
Q-score: 0.494
Kang JY, Llewellyn E, Chen J, Olinares PDB, Brewer J, Chait BT, Campbell EA, Darst SA
eLife (2021) 10 [ Pubmed: 33480355 DOI: doi:10.7554/eLife.62117 ]
- Transcription-repair-coupling factor (130 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Rna (20-mer) (6 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Escherichia coli mfd-rna polymerase elongation complex: state v (570 kDa, Complex from Escherichia coli)