Structure of E. coli dGTPase bound to T7 bacteriophage protein Gp1.2
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7u65
Q-score: 0.551
Klemm BP, Singh D, Smith CE, Hsu AL, Dillard LB, Krahn JM, London RE, Mueller GA, Borgnia MJ, Schaaper RM
PNAS (2022) 119 pp. e2123092119-e2123092119 [ DOI: doi:10.1073/pnas.2123092119 Pubmed: 36067314 ]
- Gene 1.2 protein (Complex from Escherichia phage T7)
- Dgtp triphosphohydrolase (Complex from Escherichia coli str. K-12 substr. MG1655)
- Inhibitor of dgtpase (10 kDa, Protein from Escherichia phage T7)
- Dgtpase hexamer bound to six copies of gp1.2 (Complex from Escherichia coli str. K-12 substr. MG1655)
- Deoxyguanosinetriphosphate triphosphohydrolase (59 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
Structure of E. coli dGTPase bound to T7 bacteriophage protein Gp1.2 and dGTP
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7u66
Q-score: 0.539
Klemm BP, Singh D, Smith CE, Hsu AL, Dillard LB, Krahn JM, London RE, Mueller GA, Borgnia MJ, Schaaper RM
PNAS (2022) 119 pp. e2123092119-e2123092119 [ DOI: doi:10.1073/pnas.2123092119 Pubmed: 36067314 ]
- Dgtpase hexamer bound to six copies of gp1.2 (Complex from Escherichia coli str. K-12 substr. MG1655)
- Inhibitor of dgtpase (10 kDa, Protein from Escherichia phage T7)
- Deoxyguanosinetriphosphate triphosphohydrolase (59 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Gene 1.2 protein (Complex from Escherichia phage T7)
- 2'-deoxyguanosine-5'-triphosphate (507 Da, Ligand)
- Dgtp triphosphohydrolase (Complex from Escherichia coli str. K-12 substr. MG1655)
- Magnesium ion (24 Da, Ligand)
Structure of E. coli dGTPase bound to T7 bacteriophage protein Gp1.2 and GTP
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7u67
Q-score: 0.588
Klemm BP, Singh D, Smith CE, Hsu AL, Dillard LB, Krahn JM, London RE, Mueller GA, Borgnia MJ, Schaaper RM
PNAS (2022) 119 pp. e2123092119-e2123092119 [ DOI: doi:10.1073/pnas.2123092119 Pubmed: 36067314 ]
- Dgtpase hexamer bound to six copies of gp1.2 (Complex from Escherichia coli str. K-12 substr. MG1655)
- Inhibitor of dgtpase (10 kDa, Protein from Escherichia phage T7)
- Deoxyguanosinetriphosphate triphosphohydrolase (59 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Gene 1.2 protein (Complex from Escherichia phage T7)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dgtp triphosphohydrolase (Complex from Escherichia coli str. K-12 substr. MG1655)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of Escherichia coli RNA polymerase bound to T7A1 promoter DNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7mkj
Q-score: 0.509
Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2112877118 Pubmed: 34599106 ]
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Escherichia coli rna polymerase bound to t7a1 promoter dna (Complex)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Nontemplate strand of t7a1 promoter dna (28 kDa, DNA from Escherichia phage T7)
- Template strand of t7a1 promoter dna (27 kDa, DNA from Escherichia phage T7)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna polymerase (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- T7a1 promoter dna (Complex from Escherichia phage T7)
Cryo-EM structure of T7 bacteriophage DNA translocation gp15 core protein intermediate assembly
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6ysz
Q-score: 0.434
Perez-Ruiz M, Pulido-Cid M, Luque-Ortega JR, Valpuesta JM, Cuervo A, Carrascosa JL
PNAS (2021) 118 [ Pubmed: 34417311 DOI: doi:10.1073/pnas.2026719118 ]
- Internal virion protein gp15 (88 kDa, Protein from Escherichia phage T7)
- Gp15 tubular core protein (543 kDa, Complex from Escherichia phage T7)
Cryo-EM structure of T7 bacteriophage DNA translocation gp15-gp16 core complex intermediate assembly
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6yt5
Q-score: 0.517
Perez-Ruiz M, Pulido-Cid M, Luque-Ortega JR, Valpuesta JM, Cuervo A, Carrascosa JL
PNAS (2021) 118 [ Pubmed: 34417311 DOI: doi:10.1073/pnas.2026719118 ]
- Internal virion protein gp15 (88 kDa, Protein from Escherichia phage T7)
- Gp15-gp16 core complex (1 MDa, Complex from Escherichia phage T7)
- Peptidoglycan transglycosylase gp16 (146 kDa, Protein from Escherichia phage T7)