Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 7f0r
Q-score: 0.16
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ Pubmed: 35859063 DOI: doi:10.1038/s41467-022-31871-7 ]
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Pseudomonas aeruginosa suta transcription activation complex (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (39 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Zinc ion (65 Da, Ligand)
- Pseudomonas aeruginosa suta transcription activation (Complex)
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
FtsK motor domain with dsDNA, translocating state
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 6t8b
Q-score: 0.466
Jean NL, Rutherford TJ, Lowe J
PNAS (2020) 117 pp. 14202-14208 [ Pubmed: 32513722 DOI: doi:10.1073/pnas.2001324117 ]
- Ftsk motor domain (Complex from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna (Complex from synthetic construct)
- Dsdna substrate (6 kDa, DNA from synthetic construct)
- Ftsk motor domain with dsdna, translocating state (Complex)
- Dna translocase ftsk (54 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Stalled FtsK motor domain bound to dsDNA
Resolution: 4.34 Å
EM Method: Single-particle
Fitted PDBs: 6t8g
Q-score: 0.386
Jean NL, Rutherford TJ, Lowe J
PNAS (2020) 117 pp. 14202-14208 [ Pubmed: 32513722 DOI: doi:10.1073/pnas.2001324117 ]
- Ftsk motor domain with dsdna, stalled state (Complex)
- Dna translocase ftsk (54 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna (Complex from synthetic construct)
- Ftsk motor domain (Complex from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dsdna substrate (4 kDa, DNA from synthetic construct)
Stalled FtsK motor domain bound to dsDNA end
Resolution: 3.99 Å
EM Method: Single-particle
Fitted PDBs: 6t8o
Q-score: 0.418
Jean NL, Rutherford TJ, Lowe J
PNAS (2020) 117 pp. 14202-14208 [ Pubmed: 32513722 DOI: doi:10.1073/pnas.2001324117 ]
- Dna translocase ftsk (54 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna (Complex from synthetic construct)
- Ftsk motor domain (Complex from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Ftsk motor domain at dsdna end, stalled state (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dsdna substrate (4 kDa, DNA from synthetic construct)