Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex
Resolution: 5.8 Å
EM Method: Single-particle
Fitted PDBs: 7f0r
Q-score: 0.16
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ Pubmed: 35859063 DOI: doi:10.1038/s41467-022-31871-7 ]
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna (70-mer) (21 kDa, DNA from synthetic construct)
- Dna (Complex)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Pseudomonas aeruginosa suta transcription activation complex (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (39 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
- Zinc ion (65 Da, Ligand)
- Pseudomonas aeruginosa suta transcription activation (Complex)
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1))
Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 7vf9
Q-score: 0.373
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ Pubmed: 35859063 DOI: doi:10.1038/s41467-022-31871-7 ]
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- Pseudomonas aeruginosa rnap sigmas holoenzyme complexes (432 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 7xl3
Q-score: 0.468
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ DOI: doi:10.1038/s41467-022-31871-7 Pubmed: 35859063 ]
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa rnap sigmas holoenzyme with transcription factor suta complex(open lobe) (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7xl4
Q-score: 0.39
He D, You L, Wu X, Shi J, Wen A, Yan Z, Mu W, Fang C, Feng Y, Zhang Y
Nat Commun (2022) 13 pp. 4204-4204 [ Pubmed: 35859063 DOI: doi:10.1038/s41467-022-31871-7 ]
- Transcriptional factor suta (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta (151 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor rpos (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit beta' (156 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Dna-directed rna polymerase subunit alpha (38 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- Pseudomonas aeruginosa rnap sigmas holoenzyme with transcription factor suta complex(closed lobe) (433 kDa, Complex from Pseudomonas aeruginosa PAO1)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7unq
Q-score: 0.472
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Compact if2-gdp bound to the pseudomonas aeruginosa 70s ribosome initiation complex, from focused classification and refinement (i-a) (Complex from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7unr
Q-score: 0.561
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Mrna (7 kDa, RNA from Bacteriophage sp.)
- 30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa 70s ribosome initiation complex bound to compact if2-gdp (composite structure i-a) (Complex from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Water (18 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7unt
Q-score: 0.45
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Compact if2-gdp bound to the pseudomonas aeruginosa 70s ribosome initiation complex, from focused classification and refinement (i-b) (Complex from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7unu
Q-score: 0.577
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Mrna (7 kDa, RNA from Bacteriophage sp.)
- Pseudomonas aeruginosa 70s ribosome initiation complex bound to compact if2-gdp (composite structure i-b) (Complex from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Water (18 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-A)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7unv
Q-score: 0.554
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Mrna (7 kDa, RNA from Bacteriophage sp.)
- 30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa 70s ribosome initiation complex bound to if2-gdpcp (structure ii-a) (Complex from Pseudomonas aeruginosa PAO1)
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Water (18 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)
Pseudomonas aeruginosa 70S ribosome initiation complex bound to IF2-GDPCP (structure II-B)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7unw
Q-score: 0.554
Basu RS, Sherman MB, Gagnon MG
Nat Commun (2022) 13 pp. 3388- [ DOI: doi:10.1038/s41467-022-31129-2 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l36 (4 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s14 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l31 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l15 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Mrna (7 kDa, RNA from Bacteriophage sp.)
- 30s ribosomal protein s21 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s6 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l32 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s3 (25 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l28 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s17 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s9 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l34 (5 kDa, Protein from Pseudomonas aeruginosa PAO1)
- N-formylmethionine (177 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s8 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l23 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l19 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l29 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l13 (16 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Translation initiation factor if-2 (91 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 30s ribosomal protein s13 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 16s ribosomal rna (497 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s20 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l6 (19 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s19 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s7 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l4 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l10 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l11 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s2 (27 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s15 (10 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s18 (8 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l3 (22 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l18 (12 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l9 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Pseudomonas aeruginosa 70s ribosome initiation complex bound to if2-gdpcp (structure ii-b) (Complex from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l24 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 23s ribosomal rna (937 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l27 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 30s ribosomal protein s16 (9 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l16 (15 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l35 (7 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l22 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- Water (18 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l25 (21 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l33 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l14 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l30 (6 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l21 (11 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l17 (14 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l20 (13 kDa, Protein from Pseudomonas aeruginosa PAO1)
- 5s ribosomal rna (38 kDa, RNA from Pseudomonas aeruginosa PAO1)
- 50s ribosomal protein l5 (20 kDa, Protein from Pseudomonas aeruginosa PAO1)