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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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initial_angle_assignment_type:"MAXIMUM LIKELIHOOD" AND xref_links:"pdb" AND natural_source_ncbi_code:"559292" AND author_name:"Kim HJ" AND assembly_molecular_weight:{250000 TO 500000] AND xref_links:"pdbekb" AND author_name:"Murakami K"

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EMD-22587

Download Map 3D View

Structure of TFIIH in TFIIH/Rad4-Rad23-Rad33 DNA opening complex

Release date: July 28, 2021
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7k01
Q-score: 0.331

van Eeuwen T, Shim Y, Kim HJ, Zhao T, Basu S, Garcia BA, Kaplan CD, Min JH, Murakami K

Nat Commun (2021) 12 pp. 3338-3338 [ DOI: doi:10.1038/s41467-021-23684-x Pubmed: 34099686 ]

Sample:

Dna repair helicase rad25 (95 kDa, Protein from Baker's yeast)
Dna repair helicase rad3 (89 kDa, Protein from Baker's yeast)
Iron/sulfur cluster (351 Da, Ligand)
Rna polymerase ii transcription factor b subunit 5 (8 kDa, Protein from Baker's yeast)
General transcription and dna repair factor iih subunit tfb4 (37 kDa, Protein from Baker's yeast)
Ternary complex of tfiih/rad4-rad23-rad33 with aaf damaged dna (420 MDa, Complex)
General transcription and dna repair factor iih subunit tfb2 (58 kDa, Protein from Baker's yeast)
General transcription and dna repair factor iih subunit ssl1 (52 kDa, Protein from Baker's yeast)
Zinc ion (65 Da, Ligand)
General transcription and dna repair factor iih subunit tfb1 (72 kDa, Protein from Baker's yeast)

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