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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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initial_angle_assignment_type:"MAXIMUM LIKELIHOOD" AND xref_links:"pdb" AND natural_source_ncbi_code:"559292" AND xref_links:"pdbekb" AND author_orcid:"0000-0003-2176-8337"

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EMD-10595

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Cryo-EM structure of the RNA Polymerase III-Maf1 complex

Release date: Feb. 19, 2020
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 6tut
Q-score: 0.49

Vorlander MK, Baudin F, Moir RD, Wetzel R, Hagen WJH, Willis IM, Muller CW

Nat Struct Mol Biol (2020) 27 pp. 229-232 [ Pubmed: 32066962 DOI: doi:10.1038/s41594-020-0383-y ]

Sample:

Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae S288C)
Rna polymerase iii - maf1 complex (720 kDa, Complex)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Repressor of rna polymerase iii transcription maf1 (Complex from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae S288C)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii (Complex from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Repressor of rna polymerase iii transcription maf1,repressor of rna polymerase iii transcription maf1 (30 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae S288C)

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EMD-10038

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RNA Polymerase I Open Complex conformation 2

Release date: Dec. 11, 2019
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rwe
Q-score: 0.112

Sadian Y, Baudin F, Tafur L, Murciano B, Wetzel R, Weis F, Muller CW

Nat Commun (2019) 10 pp. 5543-5543 [ Pubmed: 31804486 DOI: doi:10.1038/s41467-019-13510-w ]

Sample:

Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase i-specific transcription initiation factor rrn6 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase i (Complex from Saccharomyces cerevisiae)
Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase i subunit rpa34 (26 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase i-specific transcription initiation factor rrn7 (60 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase i-specific transcription initiation factor rrn11 (59 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nontemplate strand (22 kDa, DNA from synthetic construct)
Dna-directed rna polymerase i subunit rpa49 (46 kDa, Protein from Saccharomyces cerevisiae)
Dna (Complex from synthetic construct)
Template strand (21 kDa, DNA from synthetic construct)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae)
Rna polymerase i open complex conformation 2 (Complex)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae)
Zinc ion (65 Da, Ligand)
Rna polymerase i-specific transcription initiation factor rrn3 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription initiation factors (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae)

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EMD-4571

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Elongator catalytic subcomplex Elp123 lobe

Release date: July 17, 2019
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6qk7
Q-score: 0.416

Dauden MI, Jaciuk M, Weis F, Lin TY, Kleindienst C, Abbassi NEH, Khatter H, Krutyholowa R, Breunig KD, Kosinski J, Muller CW, Glatt S

Sci Adv (2019) 5 pp. eaaw2326-eaaw2326 [ DOI: doi:10.1126/sciadv.aaw2326 Pubmed: 31309145 ]

Sample:

Iron/sulfur cluster (351 Da, Ligand)
Elongator catalytic subcomplex elp123 (621 kDa, Complex from Saccharomyces cerevisiae)
5'-deoxyadenosine (251 Da, Ligand)
Elongator complex protein 3 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Elongator complex protein 2 (89 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Elongator complex protein 1 (153 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-0238

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Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP

Release date: April 3, 2019
Resolution: 3.42 Å
EM Method: Single-particle
Fitted PDBs: 6hko
Q-score: 0.478

Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW

eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]

Sample:

Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa49 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa34 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast rna polymerase i elongation complex bound to nucleotide analog gmpcpp (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-0239

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Yeast RNA polymerase I* elongation complex bound to nucleotide analog GMPCPP

Release date: April 3, 2019
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6hlq
Q-score: 0.524

Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW

eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]

Sample:

Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast rna polymerase i* elongation complex bound to nucleotide analog gmpcpp (Complex)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
Yeast rna polymerase i* (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-0240

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Yeast RNA polymerase I elongation complex bound to nucleotide analog GMPCPP (core focused)

Release date: April 3, 2019
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6hlr
Q-score: 0.512

Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW

eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]

Sample:

Dna-directed rna polymerase i subunit rpa190 (186 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna (6 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast rna polymerase i elongation complex bound to nucleotide analog gmpcpp (core focused) (Complex)
Dna-directed rna polymerase i subunit rpa43 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast rna polymerase i elongation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase i subunit rpa12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template strand (11 kDa, DNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerase i subunit rpa14 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase i subunit rpa135 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

[Show more]

EMD-0241

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Yeast apo RNA polymerase I*

Release date: April 3, 2019
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 6hls
Q-score: 0.515

Tafur L, Sadian Y, Hanske J, Wetzel R, Weis F, Muller CW

eLife (2019) 8 [ DOI: doi:10.7554/eLife.43204 Pubmed: 30913026 ]

Sample:

[Show more]

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EMDB [7]

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