Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ATPgS, state III (composite map)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7pt6
Q-score: 0.525
Saleh A, Noguchi Y, Aramayo R, Ivanova ME, Stevens KM, Montoya A, Sunidhi S, Carranza NL, Skwark MJ, Speck C
Nat Commun (2022) 13 pp. 2915-2915 [ DOI: doi:10.1038/s41467-022-30576-1 Pubmed: 35614055 ]
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Undefined mcm4 flexible n-terminal tail (358 Da, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Mcm2-7 double hexamer bound to two copies of cdc7-dbf4 (1 MDa, Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Cell division control protein 7 (58 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ddk kinase regulatory subunit dbf4 (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of MCM2-7 DH complexed with Cdc7-Dbf4 in the presence of ADP:BeF3, state I
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7pt7
Q-score: 0.445
Saleh A, Noguchi Y, Aramayo R, Ivanova ME, Stevens KM, Montoya A, Sunidhi S, Carranza NL, Skwark MJ, Speck C
Nat Commun (2022) 13 pp. 2915-2915 [ Pubmed: 35614055 DOI: doi:10.1038/s41467-022-30576-1 ]
- Cell division control protein 7 (58 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mcm2-7 double hexamer bound to one copy of cdc7-dbf4 (1 MDa, Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Beryllium trifluoride ion (66 Da, Ligand)
- Ddk kinase regulatory subunit dbf4 (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Undefined mcm4 flexible n-terminal tail (358 Da, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
3.0 A resolution structure of a DNA-loaded MCM double hexamer
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7p30
Q-score: 0.538
Greiwe JF, Miller TCR, Locke J, Martino F, Howell S, Schreiber A, Nans A, Diffley JFX, Costa A
Nat Struct Mol Biol (2022) 29 pp. 10-20 [ DOI: doi:10.1038/s41594-021-00698-z Pubmed: 34963704 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae mcm double hexamer bound to duplex dna (Complex from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae S288C)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))