Electron Microscopy Data Bank

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EMD-11422

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E. coli 70S-RNAP expressome complex in uncoupled state 1

Release date: Sept. 23, 2020
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zto
Q-score: 0.546

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase within the uncoupled e. coli expressome, state 1 (Complex from Escherichia coli)
Synthetic mrna and template dna (Complex from synthetic construct)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
Mrna (17 kDa, RNA from synthetic construct)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
Uncoupled e. coli expressome, state 1 (Complex)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
70s ribosome within the uncoupled e. coli expressome, state 1 (Complex from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)

[Show more]

EMD-11418

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E. coli 70S-RNAP expressome complex in NusG-coupled state (38 nt intervening mRNA)

Release date: Sept. 16, 2020
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6ztj
Q-score: 0.415

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Synthetic mrna and template dna (Complex from synthetic construct)
Dna-directed rna polymerase within the nusg-coupled expressome (Complex from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
Mrna (17 kDa, RNA from synthetic construct)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
70s ribosome within the nusg-coupled expressome (Complex from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
E. coli 70s-rnap expressome complex in nusg-coupled state (Complex)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)

[Show more]

EMD-11419

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E. coli 70S-RNAP expressome complex in collided state bound to NusG

Release date: Sept. 16, 2020
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6ztl
Q-score: 0.415

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
Mrna (15 kDa, RNA from synthetic construct)
Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase within the collided e. coli expressome (Complex from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
Synthetic mrna and template dna (Complex from synthetic construct)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
70s ribosome within the collided e. coli expressome (Complex from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
E. coli 70s-rnap expressome complex in collided state bound to nusg (Complex)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)

[Show more]

EMD-11420

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E. coli 70S-RNAP expressome complex in collided state without NusG

Release date: Sept. 16, 2020
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6ztm
Q-score: 0.437

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
Synthetic mrna and template dna strands (Complex from synthetic construct)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Mrna (18 kDa, RNA from synthetic construct)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase within the e. coli 70s-rnap expressome complex in collided state without nusg (Complex from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
Uncoupled e. coli expressome, state 1 (Complex)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
70s ribosome within the e. coli 70s-rnap expressome complex in collided state without nusg (Complex from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)

[Show more]

EMD-11421

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E. coli 70S-RNAP expressome complex in NusG-coupled state (42 nt intervening mRNA)

Release date: Sept. 16, 2020
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6ztn
Q-score: 0.365

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
70s ribosome within the mf42-nusg expressome (Complex from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
Mrna (18 kDa, RNA from synthetic construct)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase within the mf42-nusg expressome (Complex from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
Synthetic mrna and template dna (Complex from synthetic construct)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
E. coli 70s-rnap expressome complex in coupled state (42 nt intervening mrna) (Complex)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)

[Show more]

EMD-11423

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E. coli 70S-RNAP expressome complex in uncoupled state 6

Release date: Sept. 16, 2020
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6ztp
Q-score: 0.546

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase within the uncoupled e. coli expressome, state 6 (Complex from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
Synthetic mrna and template dna strands (Complex from synthetic construct)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
Mrna (17 kDa, RNA from synthetic construct)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
70s ribosome within the uncoupled e. coli expressome, state 6 (Complex from Escherichia coli)
Uncoupled e. coli expressome, state 6 (Complex)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)

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EMD-11426

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Uncoupled E. coli expressome, state 2

Release date: Sept. 16, 2020
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zu1
Q-score: 0.546

Webster MW, Takacs M, Zhu C, Vidmar V, Eduljee A, Abdelkareem M, Weixlbaumer A

Science (2020) 369 pp. 1355-1359 [ DOI: doi:10.1126/science.abb5036 Pubmed: 32820062 ]

Sample:

Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
Non-template dna strand (12 kDa, DNA from synthetic construct)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
Uncoupled e. coli expressome, state 2 (Complex)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
Template dna strand (11 kDa, DNA from synthetic construct)
Dna-directed rna polymerase within the uncoupled e. coli expressome, state 2 (Complex from Escherichia coli)
Synthetic mrna and template dna (Complex from synthetic construct)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
Mrna (17 kDa, RNA from synthetic construct)
Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
70s ribosome within the uncoupled e. coli expressome, state 2 (Complex from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
Phenylalanine (165 Da, Ligand)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)

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