The cryo-EM structure of the H2A.Z-H3.3 double-variant nucleosome
Jung H, Sokolova V, Lee G, Stevens VR, Tan D
Epigenomes (2024) [ DOI: doi:10.3390/epigenomes8020021 ]
- Histone h4 (11 kDa, Protein from Xenopus laevis)
- Nucleosome containing histone variants h3.3 and h2a.z (288 kDa, Complex from Escherichia coli)
- Dna (128-mer) (45 kDa, DNA from synthetic construct)
- Dna (128-mer) (51 kDa, DNA from synthetic construct)
- Histone h3.3 (15 kDa, Protein from Homo sapiens)
- Histone h2b 1.1 (13 kDa, Protein from Xenopus laevis)
- Histone h2a.z (13 kDa, Protein from Mus musculus)
162bp CX3CR1 nucleosome (further classified with better nucleosome end)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 8evg
Q-score: 0.531
To Be Published
- Dna (162-mer) (50 kDa, DNA from Mus musculus)
- Free cx3cr1 nucleosome (Complex from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h2a type 2-c (14 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
- Dna (162-mer) (49 kDa, DNA from Mus musculus)
- Single-chain variable fragment (29 kDa, Protein from Escherichia coli)
CX3CR1 nucleosome and wild type PU.1 complex
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8evh
Q-score: 0.509
Lian T, Guan R, Zhou BR, Bai Y
Nat Struct Mol Biol (2024) 31 pp. 633-643 [ DOI: doi:10.1038/s41594-023-01189-z Pubmed: 38267599 ]
- Dna (162-mer) (50 kDa, DNA from Mus musculus)
- Single-chain variable fragment (29 kDa, Protein from Escherichia coli)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (162-mer) (49 kDa, DNA from Mus musculus)
- Histone h2a type 2-c (14 kDa, Protein from Homo sapiens)
- Transcription factor pu.1 (32 kDa, Protein from Mus musculus)
- Nucleosome pu.1 complex (Complex from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
CX3CR1 nucleosome and PU.1 complex containing disulfide bond mutations
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8evi
Q-score: 0.559
Lian T, Guan R, Zhou BR, Bai Y
Nat Struct Mol Biol (2024) 31 pp. 633-643 [ DOI: doi:10.1038/s41594-023-01189-z Pubmed: 38267599 ]
- Single-chain variable fragment (29 kDa, Protein from Escherichia coli)
- Dna (167-mer) (51 kDa, DNA from Mus musculus)
- Transcription factor pu.1 (32 kDa, Protein from Mus musculus)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Dna (167-mer) (51 kDa, DNA from Mus musculus)
- Nucleosome pu.1 mutant complex (Complex from Homo sapiens)
- Histone h2a type 2-c (14 kDa, Protein from Homo sapiens)
- Histone h2a type 2-c (14 kDa, Protein from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
CX3CR1 nucleosome bound PU.1 and C/EBPa
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8evj
Q-score: 0.31
Lian T, Guan R, Zhou BR, Bai Y
Nat Struct Mol Biol (2024) 31 pp. 633-643 [ DOI: doi:10.1038/s41594-023-01189-z Pubmed: 38267599 ]
- Scfv (29 kDa, Protein from Escherichia coli)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Nucleosome complexed with pu.1 and c/ebpa (Complex from Mus musculus)
- Histone h2a type 2-c (14 kDa, Protein from Homo sapiens)
- Transcription factor pu.1 (32 kDa, Protein from Mus musculus)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Histone h2b type 2-e (13 kDa, Protein from Homo sapiens)
- Dna (167-mer) (51 kDa, DNA from Mus musculus)
- Dna (167-mer) (51 kDa, DNA from Mus musculus)