Cryo-EM structure of E. coli cytochrome bo3 quinol oxidase assembled in peptidiscs
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8qqk
Q-score: 0.594
Gao Y, Zhang Y, Hakke S, Mohren R, Sijbers LJPM, Peters PJ, Ravelli RBG
Biochim Biophys Acta Bioenerg (2024) 1865 pp. 149045-149045 [ Pubmed: 38614453 DOI: doi:10.1016/j.bbabio.2024.149045 ]
- Copper (ii) ion (63 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Cytochrome bo(3) ubiquinol oxidase subunit 3 (22 kDa, Protein from Escherichia coli BL21(DE3))
- Cytochrome bo(3) ubiquinol oxidase subunit 4 (12 kDa, Protein from Escherichia coli BL21(DE3))
- Cytochrome bo(3) ubiquinol oxidase subunit 2 (34 kDa, Protein from Escherichia coli BL21(DE3))
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Heme o (838 Da, Ligand)
- Cytochrome bo(3) ubiquinol oxidase subunit 1 (74 kDa, Protein from Escherichia coli BL21(DE3))
- Cytochrome bo3 quinol oxidase (144 kDa, Complex from Escherichia coli)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
E. coli BAM complex (BamABCDE) wild-type
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8bwc
Q-score: 0.482
Haysom SF, Machin J, Whitehouse JM, Horne JE, Fenn K, Ma Y, El Mkami H, Bohringer N, Schaberle TF, Ranson NA, Radford SE, Pliotas C
Angew Chem Int Ed Engl (2023) 62 pp. e202218783-e202218783 [ DOI: doi:10.1002/anie.202218783 Pubmed: 37162386 ]
- Outer membrane protein assembly factor bame (11 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (39 kDa, Protein from Escherichia coli BL21(DE3))
- Outer membrane protein assembly factor bama, bamc, bamd and bame (Complex from Escherichia coli)
- Outer membrane protein assembly factor bama (88 kDa, Protein from Escherichia coli)
- E. coli bam complex (bamabcde) wild-type (200 kDa, Complex)
- Outer membrane protein assembly factor bamd (25 kDa, Protein from Escherichia coli)
- Outer membrane protein assembly factor bamb (Complex from Escherichia coli BL21(DE3))
- Outer membrane protein assembly factor bamc (34 kDa, Protein from Escherichia coli)
Complex I from E. coli, LMNG-purified, under Turnover at pH 6, Resting state
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 7zci
Q-score: 0.637
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Flavin mononucleotide (456 Da, Ligand)
- Complex i (Complex from Escherichia coli)
- Nadh dehydrogenase subunit l (66 kDa, Protein from Escherichia coli)
- Eicosane (282 Da, Ligand)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh dehydrogenase i subunit m (56 kDa, Protein from Escherichia coli)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh-quinone oxidoreductase subunit k (10 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit h (36 kDa, Protein from Escherichia coli)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-quinone oxidoreductase subunit b (25 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit i (20 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit f (49 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit c/d (68 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase (100 kDa, Protein from Escherichia coli)
- Nadh dehydrogenase i subunit e (18 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit j (19 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit n (52 kDa, Protein from Escherichia coli)
- Nadh-quinone oxidoreductase subunit a (16 kDa, Protein from Escherichia coli)
- Calcium ion (40 Da, Ligand)