Cryo-EM structure of Escherichia coli RNA polymerase bound to lambda PR promoter DNA (class 1)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7mkd
Q-score: 0.495
Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2112877118 Pubmed: 34599106 ]
- Escherichia coli sigma 70 rna polymerase bound to lambda pr promoter dna (Complex)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Template strand of lambda pr promoter dna (27 kDa, DNA from Escherichia virus Lambda)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Lambda pr promoter dna (Complex from Escherichia virus Lambda)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Rna polymerase (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Nontemplate strand of lambda pr promoter dna (27 kDa, DNA from Escherichia virus Lambda)
Cryo-EM structure of Escherichia coli RNA polymerase bound to lambda PR promoter DNA (class 2)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7mke
Q-score: 0.412
Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2112877118 Pubmed: 34599106 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Lambda pr promoter dna (class 2) (Complex from Escherichia virus Lambda)
- Rna polymerase (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Template strand of lambda pr dna promoter (27 kDa, DNA from Escherichia virus Lambda)
- Magnesium ion (24 Da, Ligand)
- Escherichia coli rna polymerase bound to lambda pr promoter dna (Complex)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Nontemplate strand of lambda pr dna promoter (27 kDa, DNA from Escherichia virus Lambda)
Cryo-EM structure of Escherichia coli RNA polymerase bound to lambda PR (-5G to C) promoter DNA
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7mki
Q-score: 0.492
Saecker RM, Chen J, Chiu CE, Malone B, Sotiris J, Ebrahim M, Yen LY, Eng ET, Darst SA
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2112877118 Pubmed: 34599106 ]
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Rna polymerase (Complex from Escherichia coli)
- Template strand of lambda pr promoter dna (-5c to g) (27 kDa, DNA from Escherichia virus Lambda)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli)
- Chapso (631 Da, Ligand)
- Lambda pr (-5g to c) promoter dna (Complex from Escherichia virus Lambda)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Nontemplate strand of lambda pr dna promoter (-5g to c) (27 kDa, DNA from Escherichia virus Lambda)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Escherichia coli rna polymerase bound to lambda pr promoter dna (-5g to c) (Complex)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6bbm
Q-score: 0.381
Chase J, Catalano A, Noble AJ, Eng ET, Olinares PD, Molloy K, Pakotiprapha D, Samuels M, Chait B, des Georges A, Jeruzalmi D
eLife (2018) 7 [ Pubmed: 30582519 DOI: doi:10.7554/eLife.41140 ]
- Replicative dna helicase (52 kDa, Protein from Escherichia coli O111:NM)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Lambda p helicase loader (Complex from Enterobacteria phage lambda)
- Dnab helicase - lambda p helicase loader dna replication complex (Complex from Escherichia coli)
- Replication protein p (23 kDa, Protein from Escherichia phage lambda)
- E coli dnab helicase (Complex from Escherichia coli)