Cryo-EM structure of the AsCas12f-sgRNA-target DNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8j12
Q-score: 0.479
Hino T, Omura NS, Nakagawa R, Togashi T, Takeda NS, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro H, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Siksnys V, Ohmori T, Hoshino A, Nureki O
To Be Published
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Ascas12f-sgrna-target dna (Complex from Mycolicibacterium mucogenicum)
- Magnesium ion (24 Da, Ligand)
- Dna (38-mer) (11 kDa, DNA from Acidibacillus sulfooxidans)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Acidibacillus sulfuroxidans)
- Zinc ion (65 Da, Ligand)
- Rna (247-mer) (72 kDa, RNA from Acidibacillus sulfooxidans)
Cryo-EM structure of the AsCas12f-YHAM-sgRNAS3-5v7-target DNA
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8j1j
Q-score: 0.511
Hino T, Omura NS, Nakagawa R, Togashi T, Takeda NS, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro H, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Siksnys V, Ohmori T, Hoshino A, Nureki O
To Be Published
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-yham-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
Cryo-EM structure of the AsCas12f-HKRA-sgRNAS3-5v7-target DNA
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 8j3r
Q-score: 0.174
Hino T, Omura NS, Nakagawa R, Togashi T, Takeda NS, Hiramoto T, Tasaka S, Hirano H, Tokuyama T, Uosaki H, Ishiguro H, Yamano H, Ozaki Y, Motooka D, Mori H, Kirita Y, Kise Y, Itoh Y, Matoba S, Aburatani H, Yachie N, Siksnys V, Ohmori T, Hoshino A, Nureki O
To Be Published
- Dna (37-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-hkra-sgrnas3-5v7-target dna (Complex from Sulfoacidibacillus thermotolerans)
- Rna (118-mer) (38 kDa, RNA from Sulfoacidibacillus thermotolerans)
- Ascas12f-hkra (Complex from Sulfoacidibacillus thermotolerans)
- Rna (Complex)
- Zinc ion (65 Da, Ligand)
- Transposase is605 orfb c-terminal domain-containing protein (49 kDa, Protein from Sulfoacidibacillus thermotolerans)
- Dna (38-mer) (11 kDa, DNA from Sulfoacidibacillus thermotolerans)
- Magnesium ion (24 Da, Ligand)
- Dna (Complex from Sulfoacidibacillus thermotolerans)
Cryo-EM structure of Acidibacillus sulfuroxidans Cas12f in complex with sgRNA and target DNA
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8dzj
Q-score: 0.459
Wu T, Liu C, Zou S, Lyu R, Yang B, Yan H, Zhao M, Tang W
Nat Chem Biol (2023) 19 pp. 1384-1393 [ Pubmed: 37400536 DOI: doi:10.1038/s41589-023-01380-9 ]
- Orfb_zn_ribbon domain-containing protein (51 kDa, Protein from Acidibacillus sulfuroxidans)
- Sgrna (62 kDa, RNA from Acidibacillus sulfuroxidans)
- Non-target dna strand (12 kDa, DNA from Homo sapiens)
- Ascas12f-sgrna-dna (Complex from Acidibacillus sulfuroxidans)
- Zinc ion (65 Da, Ligand)
- Target dna strand (12 kDa, DNA from Homo sapiens)
Cryo-EM structure of the AsCas12f1-sgRNAv1-dsDNA ternary complex
Resolution: 2.69 Å
EM Method: Single-particle
Fitted PDBs: 7wju
Q-score: 0.609
Wu Z, Liu D, Pan D, Shen H, Ji Q
To Be Published
- Ascas12f1-sgrnav1-dsdna ternary complex (Complex from Acidibacillus sulfuroxidans)
- Ascas12f1 (Complex from synthetic construct)
- Non-target strand (1 kDa, DNA from synthetic construct)
- Orfb_zn_ribbon domain-containing protein (48 kDa, Protein from Acidibacillus sulfuroxidans)
- Dsdna (Complex)
- Sgrna1 (Complex from synthetic construct)
- Sgrnav1 (61 kDa, RNA from synthetic construct)
- Target strand (9 kDa, DNA from synthetic construct)