Cryo-EM structure of the whole photosynthetic complex from the green sulfur bacteria
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7z6q
Q-score: 0.493
Xie H, Lyratzakis A, Khera R, Koutantou M, Welsch S, Michel H, Tsiotis G
PNAS (2023) 120 pp. e2216734120-e2216734120 [ DOI: doi:10.1073/pnas.2216734120 Pubmed: 36693097 ]
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center (490 kDa, Complex from Chlorobaculum tepidum TLS)
- [(2~{r})-2-hexadecanoyloxy-3-[(2~{s},3~{s},4~{r},5~{r},6~{s})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-propyl] hexadecanoate (731 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a (911 Da, Ligand)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Chlorophyll a (893 Da, Ligand)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8gwa
Q-score: 0.541
Chen JH, Wang W, Wang C, Kuang T, Shen JR, Zhang X
J Integr Plant Biol (2023) 65 pp. 223-234 [ Pubmed: 36125941 DOI: doi:10.1111/jipb.13367 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Chlorophyll a ester (891 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Unkown protein (4 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a (911 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- Cardiolipin (1 kDa, Ligand)
- Ric1 protein (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Calcium ion (40 Da, Ligand)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Gsbrc-2fmo complex (Complex from Chlorobaculum tepidum TLS)
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO1)
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7uea
Q-score: 0.406
Puskar R, Du Truong C, Swain K, Chowdhury S, Chan KY, Li S, Cheng KW, Wang TY, Poh YP, Mazor Y, Liu H, Chou TF, Nannenga BL, Chiu PL
Nat Commun (2022) 13 pp. 5824-5824 [ DOI: doi:10.1038/s41467-022-33505-4 Pubmed: 36192412 ]
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- Chlorophyll a ester (891 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Iron/sulfur cluster (351 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a (911 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Photosynthetic assembly (rc-fmo1) (370 kDa, Complex from Chlorobaculum tepidum TLS)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
Photosynthetic assembly of Chlorobaculum tepidum (RC-FMO2)
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 7ueb
Q-score: 0.497
Puskar R, Du Truong C, Swain K, Chowdhury S, Chan KY, Li S, Cheng KW, Wang TY, Poh YP, Mazor Y, Liu H, Chou TF, Nannenga BL, Chiu PL
Nat Commun (2022) 13 pp. 5824-5824 [ DOI: doi:10.1038/s41467-022-33505-4 Pubmed: 36192412 ]
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Chlorophyll a ester (891 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- Iron/sulfur cluster (351 Da, Ligand)
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Pscf (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum TLS)
- Photosynthetic assembly (rc-fmo2) (504 kDa, Complex from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum TLS)
- Cytochrome c (22 kDa, Protein from Chlorobaculum tepidum TLS)
- Bacteriochlorophyll a (911 Da, Ligand)
- Psce (6 kDa, Protein from Chlorobaculum tepidum TLS)
- Calcium ion (40 Da, Ligand)
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
Cryo-EM structure of FMO-RC complex from green sulfur bacteria
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6m32
Q-score: 0.525
Chen JH, Wu H, Xu C, Liu XC, Huang Z, Chang S, Wang W, Han G, Kuang T, Shen JR, Zhang X
Science (2020) 370 [ Pubmed: 33214250 DOI: doi:10.1126/science.abb6350 ]
- Fmo-rc of green sulfur bacteria (Complex from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- Photosystem p840 reaction center, large subunit (81 kDa, Protein from Chlorobaculum tepidum TLS)
- 1,2-distearoyl-monogalactosyl-diglyceride (787 Da, Ligand)
- P840 reaction center 17 kda protein (16 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- [(2r,3s,4s,5r,6r)-6-[(10e,12e,14e)-2,6,10,14,19,23-hexamethyl-25-(2,3,6-trimethylphenyl)pentacosa-6,8,10,12,14,16,18,20,22,24-decaen-2-yl]oxy-3,4,5-tris(oxidanyl)oxan-2-yl]methyl dodecanoate (895 Da, Ligand)
- Bacteriochlorophyll a protein (40 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- 1,2-dipalmitoyl-phosphatidyl-glycerole (722 Da, Ligand)
- Chlorophyll a ester (891 Da, Ligand)
- Photosystem p840 reaction center iron-sulfur protein (23 kDa, Protein from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS))
- Bacteriochlorophyll a isomer (911 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 2-[(1e,3e,5e,7e,9e,11e,13e,15e,17e,19e)-3,7,12,16,20,24-hexamethylpentacosa-1,3,5,7,9,11,13,15,17,19,23-undecaenyl]-1,3,4-trimethyl-benzene (532 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Bacteriochlorophyll a (911 Da, Ligand)
Resolution: 26.5 Å
EM Method: Single-particle
Fitted PDBs: 5lcb
Q-score: -0.012
Nielsen JT, Kulminskaya NV, Bjerring M, Linnanto JM, Ratsep M, Pedersen MO, Lambrev PH, Dorogi M, Garab G, Thomsen K, Jegerschold C, Frigaard NU, Lindahl M, Nielsen NC
Nat Commun (2016) 7 pp. 12454-12454 [ DOI: doi:10.1038/ncomms12454 Pubmed: 27534696 ]