Cryo-EM structure of MraY in complex with analogue 2
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9b70
Q-score: 0.561
Yamamoto K, Sato T, Hao A, Asao K, Kaguchi R, Kusaka S, Ruddarraju RR, Kazamori D, Seo K, Takahashi S, Horiuchi M, Yokota SI, Lee SY, Ichikawa S
Nat Commun (2024) 15 pp. 5085-5085 [ DOI: doi:10.1038/s41467-024-49484-7 Pubmed: 38877016 ]
- (2~{s},3~{s})-3-[(2~{s},3~{r},4~{s},5~{r})-5-(aminomethyl)-3,4-bis(oxidanyl)oxolan-2-yl]oxy-2-[[3-[[[(2~{s})-6-azanyl-2-(hexadecanoylamino)hexanoyl]amino]methyl]phenyl]methylamino]-3-[(2~{s},3~{s},4~{r},5~{r})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]propanoic acid (934 Da, Ligand)
- Complex of mray aa dimer with nanobody bound (200 kDa, Complex from Aquifex aeolicus VF5)
- Water (18 Da, Ligand)
- Phospho-n-acetylmuramoyl-pentapeptide-transferase (40 kDa, Protein from Aquifex aeolicus VF5)
- Mrayaa nanobody (15 kDa, Protein from Lama glama)
Cryo-EM structure of MraY in complex with analogue 3
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 9b71
Q-score: 0.585
Yamamoto K, Sato T, Hao A, Asao K, Kaguchi R, Kusaka S, Ruddarraju RR, Kazamori D, Seo K, Takahashi S, Horiuchi M, Yokota SI, Lee SY, Ichikawa S
Nat Commun (2024) 15 pp. 5085-5085 [ DOI: doi:10.1038/s41467-024-49484-7 Pubmed: 38877016 ]
- Complex of mray aa dimer with nanobody bound (200 kDa, Complex from Aquifex aeolicus VF5)
- Water (18 Da, Ligand)
- Phospho-n-acetylmuramoyl-pentapeptide-transferase (40 kDa, Protein from Aquifex aeolicus VF5)
- (2~{s},3~{s})-3-[(2~{s},3~{r},4~{s},5~{r})-5-(aminomethyl)-3,4-bis(oxidanyl)oxolan-2-yl]oxy-3-[(2~{s},3~{s},4~{r},5~{r})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]-2-[[4-[[[(2~{s})-5-carbamimidamido-2-(hexadecanoylamino)pentanoyl]amino]methyl]phenyl]methylamino]propanoic acid (962 Da, Ligand)
- Mrayaa nanobody (15 kDa, Protein from Lama glama)
CryoEM structure of the A. aeolicus WzmWzt transporter bound to the native O antigen and ADP
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8dnc
Q-score: 0.464
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ Pubmed: 36064941 DOI: doi:10.1038/s41467-022-32597-2 ]
- Abc transporter (46 kDa, Protein from Aquifex aeolicus)
- O antigen abc transporter (Complex from Aquifex aeolicus)
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
CryoEM structure of the A.aeolicus WzmWzt transporter bound to ATP
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8dne
Q-score: 0.43
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ Pubmed: 36064941 DOI: doi:10.1038/s41467-022-32597-2 ]
- Abc transporter (46 kDa, Protein from Aquifex aeolicus VF5)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- O antigen abc transporter (Complex from Aquifex aeolicus VF5)
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus VF5)
CryoEM structure of the A. aeolicus WzmWzt transporter bound to ADP
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 8dou
Q-score: 0.428
Spellmon N, Muszynski A, Gorniak I, Vlach J, Hahn D, Azadi P, Zimmer J
Nat Commun (2022) 13 pp. 5226-5226 [ Pubmed: 36064941 DOI: doi:10.1038/s41467-022-32597-2 ]
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus VF5)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus VF5)
- O antigen abc transporter (Complex from Aquifex aeolicus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Mg2+/ATP-bound structure of the full-length WzmWzt O antigen ABC transporter in lipid nanodiscs
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7k2t
Q-score: 0.472
PNAS (2021) 118 [ Pubmed: 33443152 DOI: doi:10.1073/pnas.2016144118 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Transport permease protein (30 kDa, Protein from Aquifex aeolicus (strain VF5))
- Full-length wzmwzt o antigen abc transporter (152 kDa, Complex from Aquifex aeolicus VF5)
- Abc transporter (46 kDa, Protein from Aquifex aeolicus (strain VF5))