Cryo-EM structure of the Arabidopsis thaliana I+III2 supercomplex (CI membrane core)
Resolution: 2.13 Å
EM Method: Single-particle
Fitted PDBs: 8bef
Q-score: 0.729
Klusch N, Dreimann M, Senkler J, Rugen N, Kuhlbrandt W, Braun HP
Nat Plants (2023) 9 pp. 142-156 [ Pubmed: 36585502 DOI: doi:10.1038/s41477-022-01308-6 ]
- P1 (11 kDa, Protein from thale cress)
- At4g16450 (11 kDa, Protein from thale cress)
- At3g07480.1 (17 kDa, Protein from thale cress)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Butyryl coenzyme a (837 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (74 kDa, Protein from thale cress)
- Cardiolipin (1 kDa, Ligand)
- Uncharacterized protein at1g67785 (7 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (16 kDa, Protein from thale cress)
- 2,3-dimethoxy-5-methyl-6-(3,11,15,19-tetramethyl-eicosa-2,6,10,14,18-pentaenyl)-[1,4]benzoquinone (522 Da, Ligand)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
- Uncharacterized protein at2g27730, mitochondrial (11 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (7 kDa, Protein from thale cress)
- Excitatory amino acid transporter (9 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from thale cress)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 2 (55 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 4 (55 kDa, Protein from thale cress)
- Mitochondrial arabidopsis thaliana i+iii2 supercomplex (ci membrane core) (Complex from Arabidopsis t)
- Ubiquinone-9 (795 Da, Ligand)
- Gamma carbonic anhydrase 2, mitochondrial (30 kDa, Protein from thale cress)
- 2-{[(4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranosyl)oxy]methyl}-4-{[(3beta,9beta,14beta,17beta,25r)-spirost-5-en-3-yl]oxy}butyl 4-o-alpha-d-glucopyranosyl-alpha-d-glucopyranoside (1 kDa, Ligand)
- Water (18 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4l (11 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from thale cress)
- Fe (iii) ion (55 Da, Ligand)
- Gamma carbonic anhydrase 1, mitochondrial (30 kDa, Protein from thale cress)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Gamma carbonic anhydrase-like 2, mitochondrial (27 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-b (9 kDa, Protein from thale cress)
- At2g46540/f11c10.23 (6 kDa, Protein from thale cress)
- Outer envelope pore protein 16-3, chloroplastic/mitochondrial (17 kDa, Protein from thale cress)
Cryo-EM structure of Arabidopsis thaliana complex-I (open conformation)
Resolution: 3.72 Å
EM Method: Single-particle
Fitted PDBs: 7ar7
Q-score: 0.438
Klusch N, Senkler J, Yildiz O, Kuehlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ Pubmed: 33768254 DOI: doi:10.1093/plcell/koab092 ]
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2 (43 kDa, Protein from thale cress)
- Gamma carbonic anhydrase 2, mitochondrial (28 kDa, Protein from thale cress)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Unknown (2 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-a (14 kDa, Protein from thale cress)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (21 kDa, Protein from thale cress)
- Acyl carrier protein 2, mitochondrial (9 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-a (5 kDa, Protein from thale cress)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (6 kDa, Protein from thale cress)
- 1,2-dicaproyl-sn-phosphatidyl-l-serine (455 Da, Ligand)
- Gamma carbonic anhydrase 1, mitochondrial (25 kDa, Protein from thale cress)
- Transmembrane protein (8 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from thale cress)
- Phosphatidylinositol (887 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (55 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3 (22 kDa, Protein from thale cress)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial (16 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (13 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (17 kDa, Protein from thale cress)
- Excitatory amino acid transporter (8 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (12 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 (5 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5-a (7 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (9 kDa, Protein from thale cress)
- Zinc ion (65 Da, Ligand)
- At2g31490 protein (8 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from thale cress)
- At2g46540/f11c10.23 (4 kDa, Protein from thale cress)
- At4g16450 (10 kDa, Protein from thale cress)
- Ubiquinone-9 (795 Da, Ligand)
- Acyl carrier protein 1, mitochondrial (9 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial (75 kDa, Protein from thale cress)
- Gamma carbonic anhydrase-like 2, mitochondrial (23 kDa, Protein from thale cress)
- B14.5a (1 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (36 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 6 (23 kDa, Protein from thale cress)
- At1g67350 (10 kDa, Protein from thale cress)
- Uncharacterized protein at2g27730, mitochondrial (3 kDa, Protein from thale cress)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (6 kDa, Protein from thale cress)
- Phosphatidylethanolamine (734 Da, Ligand)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8-a, mitochondrial (19 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-b (11 kDa, Protein from thale cress)
- Esss subunit of nadh:ubiquinone oxidoreductase (complex i) protein (8 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (48 kDa, Protein from thale cress)
- Flavin mononucleotide (456 Da, Ligand)
- Probable nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (7 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (5 kDa, Protein from thale cress)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-b (11 kDa, Protein from thale cress)
- Cryo-em structure of arabidopsis thaliana complex-i (open conformation) (Complex from Arabidopsis thaliana)
- Nadh-ubiquinone oxidoreductase chain 2 (54 kDa, Protein from thale cress)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from thale cress)