The cryo-EM structure of A. thaliana Pol IV-RDR2 backtracked complex
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 7eu0
Q-score: 0.487
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 backtracked complex (550 kDa, Complex from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna (33-mer) (9 kDa, DNA from synthetic construct)
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Dna (5'-d(*cp*tp*gp*tp*gp*cp*tp*tp*ap*tp*cp*gp*gp*tp*ap*gp*ap*gp*tp*g)-3') (6 kDa, DNA from synthetic construct)
- Rna (5'-r(*cp*cp*gp*a)-3') (1 kDa, RNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- Rna (39-mer) (12 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
The cryo-EM structure of A. thaliana Pol IV-RDR2 holoenzyme
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 7eu1
Q-score: 0.434
Huang K, Wu XX, Fang CL, Xu ZG, Zhang HW, Gao J, Zhou CM, You LL, Gu ZX, Mu WH, Feng Y, Wang JW, Zhang Y
Science (2021) 374 pp. 1579-1586 [ Pubmed: 34941388 DOI: doi:10.1126/science.abj9184 ]
- Dna-directed rna polymerases ii, iv and v subunit 9a (13 kDa, Protein from Arabidopsis thaliana)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 11 (13 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 8b (16 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 10 (8 kDa, Protein from Arabidopsis thaliana)
- Rna-dependent rna polymerase 2 (129 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 6a (16 kDa, Protein from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerases ii, iv and v subunit 3 (35 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerase iv subunit 1 (169 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii, iv and v subunit 12 (5 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases iv and v subunit 2 (132 kDa, Protein from Arabidopsis thaliana)
- Dna-directed rna polymerases ii and iv subunit 5a (24 kDa, Protein from Arabidopsis thaliana)
- A. thaliana pol iv-rdr2 holoenzyme (Complex from Arabidopsis thaliana)
Cryo-EM structure of Arabidopsis DCL3 in complex with a 40-bp RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7vg2
Q-score: 0.545
Wang Q, Xue Y, Zhang L, Zhong Z, Feng S, Wang C, Xiao L, Yang Z, Harris CJ, Wu Z, Zhai J, Yang M, Li S, Jacobsen SE, Du J
Science (2021) 374 pp. 1152-1157 [ Pubmed: 34648373 DOI: doi:10.1126/science.abl4546 ]
- Rna (Complex)
- Dcl3 in complex with a 40-bp rna (210 kDa, Complex)
- Zinc ion (65 Da, Ligand)
- Tas1a forward strand (5'-phosphorylation) (12 kDa, RNA from Arabidopsis thaliana)
- Dicer-like 3 (182 kDa, Protein from Arabidopsis thaliana)
- Tas1a reverse strand (13 kDa, RNA from Arabidopsis thaliana)
- Dcl3 (Complex from Arabidopsis thaliana)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of Arabidopsis DCL3 in complex with a 30-bp RNA
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 7vg3
Q-score: 0.462
Wang Q, Xue Y, Zhang L, Zhong Z, Feng S, Wang C, Xiao L, Yang Z, Harris CJ, Wu Z, Zhai J, Yang M, Li S, Jacobsen SE, Du J
Science (2021) 374 pp. 1152-1157 [ DOI: doi:10.1126/science.abl4546 Pubmed: 34648373 ]
- Tas1a rna reverse strand (10 kDa, RNA from Arabidopsis thaliana)
- Dcl3 in complex with a 30-bp rna (200 kDa, Complex)
- Dcl3 (Complex from Arabidopsis thaliana)
- Zinc ion (65 Da, Ligand)
- Dicer-like 3 (182 kDa, Protein from Arabidopsis thaliana)
- Rna (Complex)
- Tas1a rna forward strand (5'-phosphorylated) (9 kDa, RNA from Arabidopsis thaliana)
- Calcium ion (40 Da, Ligand)
Ligand-triggered allosteric ADP release primes a plant NLR complex
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6j5w
Q-score: 0.452
Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW, Zhou JM, Chai J
Science (2019) 364 [ Pubmed: 30948526 DOI: doi:10.1126/science.aav5868 ]
- Rks1 (40 kDa, Protein from Arabidopsis thaliana)
- Disease resistance rpp13-like protein 4 (97 kDa, Protein from Arabidopsis thaliana)
- Zar1-rks1 with adp complex (130 kDa, Complex from Arabidopsis)
- Adenosine-5'-diphosphate (427 Da, Ligand)