Cryo-EM structure of GPI-T with a chimeric GPI-anchored protein
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 8imx
Q-score: 0.567
Xu Y, Li T, Zhou Z, Hong J, Chao Y, Zhu Z, Zhang Y, Qu Q, Li D
Nat Commun (2023) 14 pp. 5520-5520 [ Pubmed: 37684232 DOI: doi:10.1038/s41467-023-41281-y ]
- Gpi transamidase component pig-s,gfp-like fluorescent chromoprotein cfp484 (90 kDa, Protein from Clavularia sp.)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ul16-binding protein 2,gfp-like fluorescent chromoprotein cfp484 (35 kDa, Protein from Homo sapiens)
- 2-azanylethyl [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate (303 Da, Ligand)
- Met-ser-ser peptide (323 Da, Protein from synthetic construct)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- [(2r)-1-hexadecanoyloxy-3-[[3-[[(2r)-3-hexadecanoyloxy-2-[(z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (z)-octadec-9-enoate (1 kDa, Ligand)
- Calcium ion (40 Da, Ligand)
- [(2r)-1-[[(1s,2r,3r,4s,5s,6r)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate (1 kDa, Ligand)
- Gpi transamidase component pig-t,gfp-like fluorescent chromoprotein cfp484 (94 kDa, Protein from Clavularia sp.)
- Gpi-anchor transamidase,gfp-like fluorescent chromoprotein cfp484 (73 kDa, Protein from Clavularia sp.)
- Magnesium ion (24 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- Complex of gpi-t with a chimeric gpi-anchored protein (469 MDa, Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Glycosylphosphatidylinositol anchor attachment 1 protein,gfp-like fluorescent chromoprotein cfp484 (96 kDa, Protein from Clavularia sp.)
- 2-azanylethyl [(2r,3s,4s,5s,6s)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate (303 Da, Ligand)
- Phosphatidylinositol glycan anchor biosynthesis class u protein,gfp-like fluorescent chromoprotein cfp484 (77 kDa, Protein from Clavularia sp.)
- 2-amino-2-deoxy-alpha-d-glucopyranose (179 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
Cryo-EM structure of GPI-T (inactive mutant) with GPI and proULBP2, a proprotein substrate
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8imy
Q-score: 0.536
Xu Y, Li T, Zhou Z, Hong J, Chao Y, Zhu Z, Zhang Y, Qu Q, Li D
Nat Commun (2023) 14 pp. 5520-5520 [ Pubmed: 37684232 DOI: doi:10.1038/s41467-023-41281-y ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ul16-binding protein 2 (28 kDa, Protein from Homo sapiens)
- [(2r)-1-[[(1s,2r,3r,4s,5s,6r)-2-hexadecanoyloxy-3,4,5,6-tetrakis(oxidanyl)cyclohexyl]oxy-oxidanyl-phosphoryl]oxy-3-octadecoxy-propan-2-yl] (5z,8z,11z,14z)-icosa-5,8,11,14-tetraenoate (1 kDa, Ligand)
- Gpi-anchor transamidase,gfp-like fluorescent chromoprotein cfp484 (73 kDa, Protein from Clavularia sp.)
- Magnesium ion (24 Da, Ligand)
- Digitonin (1 kDa, Ligand)
- Phosphatidylinositol glycan anchor biosynthesis class u protein,gfp-like fluorescent chromoprotein cfp484 (80 kDa, Protein from Clavularia sp.)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Glycosylphosphatidylinositol anchor attachment 1 protein,gfp-like fluorescent chromoprotein cfp484 (96 kDa, Protein from Clavularia sp.)
- Complex of gpi-t (inactive mutant) with gpi and proulbp2 (463 MDa, Complex from Homo sapiens)
- 2-azanylethyl [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-2,4,5-tris(oxidanyl)oxan-3-yl] hydrogen phosphate (303 Da, Ligand)
- 2-azanylethyl [(2r,3s,4s,5s,6s)-3,4,5,6-tetrakis(oxidanyl)oxan-2-yl]methyl hydrogen phosphate (303 Da, Ligand)
- Gpi transamidase component pig-t,gfp-like fluorescent chromoprotein cfp484 (92 kDa, Protein from Clavularia sp.)
- [(2r)-1-hexadecanoyloxy-3-[[3-[[(2r)-3-hexadecanoyloxy-2-[(z)-octadec-9-enoyl]oxy-propoxy]-oxidanyl-phosphoryl]oxy-2-oxidanyl-propoxy]-oxidanyl-phosphoryl]oxy-propan-2-yl] (z)-octadec-9-enoate (1 kDa, Ligand)
- Gpi transamidase component pig-s,gfp-like fluorescent chromoprotein cfp484 (90 kDa, Protein from Clavularia sp.)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- 2-amino-2-deoxy-alpha-d-glucopyranose (179 Da, Ligand)
- Calcium ion (40 Da, Ligand)