Cryo-EM of tri-pilus from S. islandicus REY15A
Resolution: 3.47 Å
EM Method: Helical reconstruction
Fitted PDBs: 8tib
Q-score: 0.523
Eastep GN, Liu J, Rich-New ST, Egelman EH, Krupovic M, Wang F
To Be Published
- Duf973 family protein (14 kDa, Protein from Sulfolobus islandicus REY15A)
- Tri-pilus (Complex from Sulfolobus islandicus REY15A)
Cryo-EM of mono-pilus from S. islandicus REY15A
Resolution: 3.89 Å
EM Method: Helical reconstruction
Fitted PDBs: 8tif
Q-score: 0.477
Liu J, Eastep GN, Cvirkaite-Krupovic V, Rich-New ST, Kreutzberger M, Egelman EH, Krupovic M, Wang F
To Be Published
- Duf973 family protein (14 kDa, Protein from Sulfolobus islandicus REY15A)
- Mono-pilus (Complex from Sulfolobus islandicus REY15A)
Cryo-EM structure of the supercoiled S. islandicus REY15A archaeal flagellar filament
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8cwm
Q-score: 0.492
Kreutzberger MAB, Sonani RR, Liu J, Chatterjee S, Wang F, Sebastian AL, Biswas P, Ewing C, Zheng W, Poly F, Frankel G, Luisi BF, Calladine CR, Krupovic M, Scharf BE, Egelman EH
Cell (2022) 185 pp. 3487-3500.e14 [ DOI: doi:10.1016/j.cell.2022.08.009 Pubmed: 36057255 ]
- Archaeal flagellar filament (Complex from Sulfolobus islandicus)
- Flagellin (33 kDa, Protein from Sulfolobus islandicus REY15A)
Type III-B Cmr-beta Cryo-EM structure of the Apo state
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6s6b
Q-score: 0.456
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crrna (51-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Zinc ion (65 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6s8b
Q-score: 0.48
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (46-mer) (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the type III-B Cmr-beta complex bound to non-cognate target RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6s8e
Q-score: 0.397
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta complex bound to non-cognate target rna (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmr7 (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Non-cognate target rna (15 kDa, RNA from synthetic construct)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 6s91
Q-score: 0.463
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (Complex from synthetic construct)
- Crrna (48-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp proteins cmr4 and cmr6, and crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated proteins cmr5, cmr3, cmr1, cmr2, cmr7 (Complex from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex)
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6sh8
Q-score: 0.437
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Cognate target rna (Complex from synthetic construct)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 6shb
Q-score: 0.442
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crrna (49-mer) (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Manganese (ii) ion (54 Da, Ligand)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crrna (49-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (Complex from synthetic construct)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (14 kDa, RNA from synthetic construct)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 6sic
Q-score: 0.343
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)