Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7abi
Q-score: 0.145
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U6 snrna (34 kDa, RNA from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Minx m3 pre-mrna (103 kDa, RNA from synthetic construct)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dna/rna-binding protein kin17 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Beta-catenin-like protein 1 (65 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Human pre-bact-2 spliceosome (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Intron-binding protein aquarius (171 kDa, Protein from Human)
- Ring-type e3 ubiquitin-protein ligase ppil2 (58 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Human pre-bact-2 spliceosome (Complex)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
Cryo-EM dataset of human pre-Bact spliceosome
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Lührmann R
Science (2020) 370 [ Pubmed: 33243851 DOI: 10.1126/science.abc3753 ]
Image sets:
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 7abg
Q-score: 0.147
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Serine/arginine-rich splicing factor 1 (27 kDa, Protein from Homo sapiens)
- Ww domain-binding protein 11 (70 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 1 (91 kDa, Protein from Homo sapiens)
- Transcription elongation regulator 1 (124 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Nuclear cap-binding protein subunit 2 (18 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
- Pre-bact-1 spliceosomal complex (Complex)
- Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
- 7-methyl-guanosine-5'-triphosphate-5'-guanosine (803 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
Human pre-Bact-2 spliceosome core structure
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 7aav
Q-score: 0.393
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U2 snrna (60 kDa, RNA from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Pre-bact-2 spliceosomal core structure (Complex)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Pre-bact-2 spliceosomal core structure (Complex from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Minx m3 pre-mrna (Complex from synthetic construct)
- U6 snrna (34 kDa, RNA from Human)
Human pre-Bact-1 spliceosome core structure
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7abf
Q-score: 0.374
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- Inositol hexakisphosphate (660 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Minx m3 rna (Complex from synthetic construct)
- Pre-bact-1 spliceosomal complex (Complex from Homo sapiens)
- Ubiquitin-like protein 5 (8 kDa, Protein from Human)
- Ww domain-binding protein 11 (70 kDa, Protein from Human)
- Pre-bact-1 spliceosomal complex (Complex)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- U5 small nuclear rna (36 kDa, RNA from Human)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Transcription elongation regulator 1 (121 kDa, Protein from Human)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Human)
- U6 small nuclear rna (34 kDa, RNA from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Minx m3 rna (73 kDa, RNA from synthetic construct)
Human pre-Bact-2 spliceosome (SF3b/U2 snRNP portion)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7abh
Q-score: 0.356
Townsend C, Leelaram MN, Agafonov DE, Dybkov O, Will CL, Bertram K, Urlaub H, Kastner B, Stark H, Luhrmann R
Science (2020) 370 [ DOI: doi:10.1126/science.abc3753 Pubmed: 33243851 ]
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Human pre-bact-2 spliceosome (sf3b/u2 snrnp portion) (Complex)
- Minx m3 pre-mrna (73 kDa, RNA from synthetic construct)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 1 (102 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Dna/rna-binding protein kin17 (45 kDa, Protein from Homo sapiens)
- Human pre-bact-2 spliceosome (sf3b/u2 snrnp portion) (Complex from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Minx m3 pre-mrna (Complex from synthetic construct)
Human C Complex Spliceosome - High-resolution CORE
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zym
Q-score: 0.498
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
- Pre-mrna (103 kDa, RNA from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 snrna (60 kDa, RNA from Human)
- Protein frg1 (29 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from Human)
- Splicing factor yju2 (21 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Corepressor interacting with rbpj 1 (52 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (207 kDa, Protein from Human)
- Wd repeat-containing protein 70 (73 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
- Human c complex spliceosome - high resolution core (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- U6 snrna (25 kDa, RNA from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (107 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (25 kDa, Protein from Human)
Human C Complex Spliceosome - MultiBody refined EXTENDED CORE
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined BRR2/PRP16 region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined AQR/SYF1 region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]