Human C Complex Spliceosome - High-resolution CORE
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6zym
Q-score: 0.498
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
- Pre-mrna (103 kDa, RNA from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- U2 snrna (60 kDa, RNA from Human)
- Protein frg1 (29 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from Human)
- Splicing factor yju2 (21 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc25 homolog (49 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- U5 snrna (36 kDa, RNA from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Corepressor interacting with rbpj 1 (52 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (207 kDa, Protein from Human)
- Wd repeat-containing protein 70 (73 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (140 kDa, Protein from Human)
- Human c complex spliceosome - high resolution core (Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- U6 snrna (25 kDa, RNA from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (107 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor rbm22 (25 kDa, Protein from Human)
Human C Complex Spliceosome - MultiBody refined EXTENDED CORE
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined BRR2/PRP16 region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined AQR/SYF1 region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined U2 Proteins region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
Human C Complex Spliceosome - MultiBody refined PRP19/Helical-Bundle region
Bertram K, El Ayoubi L, Dybkov O, Agafonov DE, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Luhrmann R
Mol Cell (2020) 80 pp. 127-139.e6 [ Pubmed: 33007253 DOI: doi:10.1016/j.molcel.2020.09.012 ]
5'domain of human 17S U2 snRNP
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6y50
Q-score: 0.351
Zhang Z, Will CL, Bertram K, Dybkov O, Hartmuth K, Agafonov DE, Hofele R, Urlaub H, Kastner B, Luhrmann R, Stark H
Nature (2020) 583 pp. 310-313 [ DOI: doi:10.1038/s41586-020-2344-3 Pubmed: 32494006 ]
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Hiv tat-specific factor 1 (85 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Probable atp-dependent rna helicase ddx46 (117 kDa, Protein from Homo sapiens)
- Human 17s u2 snrnp (Complex from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
Resolution: 7.1 Å
EM Method: Single-particle
Fitted PDBs: 6y53
Q-score: 0.057
Zhang Z, Will CL, Bertram K, Dybkov O, Hartmuth K, Agafonov DE, Hofele R, Urlaub H, Kastner B, Luhrmann R, Stark H
Nature (2020) 583 pp. 310-313 [ Pubmed: 32494006 DOI: doi:10.1038/s41586-020-2344-3 ]
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx46 (117 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Hiv tat-specific factor 1 (85 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Human 17s u2 snrnp (Complex from Homo sapiens)
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Resolution: 5.9 Å
EM Method: Single-particle
Fitted PDBs: 5mqf
Q-score: 0.202
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lu Hrmann R
Nature (2017) 542 pp. 318-323 [ DOI: doi:10.1038/nature21079 Pubmed: 28076346 ]
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Intron-binding protein aquarius (173 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Human)
- Homo sapiens u5 a small nuclear rna (36 kDa, RNA from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Human c* spliceosome, masked refinement (Complex from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Human gene for small nuclear rna u2 (snrna u2) (60 kDa, RNA from Human)
- Minx pre-mrna (intron) (104 kDa, RNA from Human)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Human)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Snw domain-containing protein 1 (61 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Protein bud31 homolog (17 kDa, Protein from Human)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Human)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Homo sapiens rna, u6 small nuclear 1 (rnu6-1), small nuclear rna (34 kDa, RNA from Human)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Human)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Human)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Human)
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, Lu Hrmann R
Nature (2017) 542 pp. 318-323 [ DOI: doi:10.1038/nature21079 Pubmed: 28076346 ]