Resolution: 2.72 Å
EM Method: Single-particle
Fitted PDBs: 7zlg
Q-score: 0.454
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ DOI: doi:10.1038/s41589-022-01219-9 Pubmed: 36604564 ]
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- C-mannosyltransferase cedpy19, in complex with acceptor peptide and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7zlh
Q-score: 0.455
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ DOI: doi:10.1038/s41589-022-01219-9 Pubmed: 36604564 ]
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in apo state, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 7zli
Q-score: 0.496
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ DOI: doi:10.1038/s41589-022-01219-9 Pubmed: 36604564 ]
- [(2~{s},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl] [(3~{s},6~{z},10~{e},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenyl] hydrogen phosphate (602 Da, Ligand)
- C-mannosyltransferase cedpy19, in complex with dol25-p-man and bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7zlj
Q-score: 0.411
Bloch JS, John A, Mao R, Mukherjee S, Boilevin J, Irobalieva RN, Darbre T, Scott NE, Reymond JL, Kossiakoff AA, Goddard-Borger ED, Locher KP
Nat Chem Biol (2023) 19 pp. 575-584 [ Pubmed: 36604564 DOI: doi:10.1038/s41589-022-01219-9 ]
- Cmt2-fab heavy chain (25 kDa, Protein from synthetic construct)
- C-mannosyltransferase cedpy19, in ternary complex with dol25-p-c-man and acceptor peptide, bound to cmt2-fab and anti-fab nanobody (Complex from Caenorhabditis elegans)
- Cmt2-fab light chain (23 kDa, Protein from synthetic construct)
- C-mannosyltransferase dpy-19 (80 kDa, Protein from Caenorhabditis elegans)
- Water (18 Da, Ligand)
- [(2~{r},3~{s},4~{s},5~{s},6~{r})-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]methyl-[(3~{s},6~{e},10~{z},14~{e})-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraenoxy]phosphinic acid (600 Da, Ligand)
- Synthetic octapeptide wehi 1886493 (821 Da, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from synthetic construct)
Structure of yeast oligosaccharylransferase complex with lipid-linked oligosaccharide bound
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8agb
Q-score: 0.553
Ramirez AS, de Capitani M, Pesciullesi G, Kowal J, Bloch JS, Irobalieva RN, Reymond JL, Aebi M, Locher KP
Nat Commun (2022) 13 pp. 7296-7296 [ Pubmed: 36435935 DOI: doi:10.1038/s41467-022-35067-x ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit swp1 (31 kDa, Protein from Saccharomyces cerevisiae)
- Phosphatidylethanolamine (734 Da, Ligand)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Phosphono [(3~{r},6~{e},10~{e})-3,7,11,15-tetramethylhexadeca-6,10,14-trienyl] hydrogen phosphate (452 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (54 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost2 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost4 (6 kDa, Protein from Saccharomyces cerevisiae)
- Yeast ost complex with lipid-linked oligosaccharide bound (284 kDa, Complex from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost5 (9 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1 (49 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 (81 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8agc
Q-score: 0.548
Ramirez AS, de Capitani M, Pesciullesi G, Kowal J, Bloch JS, Irobalieva RN, Reymond JL, Aebi M, Locher KP
Nat Commun (2022) 13 pp. 7296-7296 [ Pubmed: 36435935 DOI: doi:10.1038/s41467-022-35067-x ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Peptide (682 Da, Protein from Saccharomyces cerevisiae)
- Yeast ost complex with lipid-linked oligosaccharide and non-acceptor peptide bound (284 kDa, Complex from Saccharomyces cerevisiae)
- Phosphono [(3~{r},6~{e},10~{e})-3,7,11,15-tetramethylhexadeca-6,10,14-trienyl] hydrogen phosphate (452 Da, Ligand)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Manganese (ii) ion (54 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (54 kDa, Protein from Saccharomyces cerevisiae)
- 2-[3,6-bis(dimethylamino)xanthen-9-yl]-5-methanoyl-benzoate (414 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (31 kDa, Protein from Saccharomyces cerevisiae)
- Ost4 isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost2 (14 kDa, Protein from Saccharomyces cerevisiae)
- Ost3 isoform 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost5 (9 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (81 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1 (49 kDa, Protein from Saccharomyces cerevisiae)
Structure of yeast oligosaccharylransferase complex with acceptor peptide bound
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8age
Q-score: 0.571
Ramirez AS, de Capitani M, Pesciullesi G, Kowal J, Bloch JS, Irobalieva RN, Reymond JL, Aebi M, Locher KP
Nat Commun (2022) 13 pp. 7296-7296 [ DOI: doi:10.1038/s41467-022-35067-x Pubmed: 36435935 ]
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1 (54 kDa, Protein from Saccharomyces cerevisiae)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit wbp1 (49 kDa, Protein from Saccharomyces cerevisiae)
- Ost3 isoform 1 (39 kDa, Protein from Saccharomyces cerevisiae)
- 2-[3,6-bis(dimethylamino)xanthen-9-yl]-5-methanoyl-benzoate (414 Da, Ligand)
- Phosphatidylethanolamine (734 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3 (81 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost2 (14 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- Peptide (696 Da, Protein from Saccharomyces cerevisiae)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- (2~{s},3~{r},4~{r},5~{s},6~{s})-2-(hydroxymethyl)-6-[(1~{s},2~{r},3~{r},4~{r},5'~{s},6~{s},7~{r},8~{s},9~{r},12~{r},13~{r},15~{s},16~{s},18~{r})-5',7,9,13-tetramethyl-3,15-bis(oxidanyl)spiro[5-oxapentacyclo[10.8.0.0^{2,9}.0^{4,8}.0^{13,18}]icosane-6,2'-oxane]-16-yl]oxy-oxane-3,4,5-triol (610 Da, Ligand)
- Ost4 isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2 (31 kDa, Protein from Saccharomyces cerevisiae)
- Manganese (ii) ion (54 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit ost5 (9 kDa, Protein from Saccharomyces cerevisiae)
- Yeast ost complex with acceptor peptide bound (284 kDa, Complex from Saccharomyces cerevisiae)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7php
Q-score: 0.505
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Multidrug resistance protein norm (52 kDa, Protein from Vibrio cholerae RC385)
- Norm-nb17_4 (14 kDa, Protein from synthetic construct)
- Water (18 Da, Ligand)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Nabfab hc (Complex from synthetic construct)
- Nabfab lc (Complex from synthetic construct)
- Anti-fab nanobody (Complex)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
- Multidrug resistance protein norm (Complex from synthetic construct)
- Norm-nb17_4 (Complex from Lama glama)
Resolution: 8.45 Å
EM Method: Single-particle
Fitted PDBs: 7phq
Q-score: 0.111
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2115435118 Pubmed: 34782475 ]
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Dmt-nb16_4 (14 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)