Cryogenic electron microscopy of somatostatin receptor 2/SST14/Gi3 complex
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) [ Pubmed: 35210615 DOI: 10.1038/s41594-022-00727-5 ]
Image sets:
Cryogenic electron microscopy of somatostatin receptor 2/Octreotide/Gi3 complex
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) [ Pubmed: 35210615 DOI: 10.1038/s41594-022-00727-5 ]
Image sets:
CryoEM structure of somatostatin receptor 2 in complex with somatostatin-14 and Gi3
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7t10
Q-score: 0.615
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 210-217 [ Pubmed: 35210615 DOI: doi:10.1038/s41594-022-00727-5 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Somatostatin receptor type 2,kappa-type opioid receptor (45 kDa, Protein from Homo sapiens)
- Somatostatin receptor 2 in complex with somatostatin-14 and gi3. (Complex)
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Somatostatin-14 (1 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
CryoEM structure of somatostatin receptor 2 in complex with Octreotide and Gi3.
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7t11
Q-score: 0.53
Robertson MJ, Meyerowitz JG, Panova O, Borrelli K, Skiniotis G
Nat Struct Mol Biol (2022) 29 pp. 210-217 [ DOI: doi:10.1038/s41594-022-00727-5 Pubmed: 35210615 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Somatostatin receptor 2 in complex with octreotide and gi3. (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Octreotide (1 kDa, Protein from synthetic construct)
- Somatostatin receptor type 2,kappa-type opioid receptor (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
Human CTPS1 bound to UTP, AMPPNP, and glutamine
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mgz
Q-score: 0.55
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2107968118 Pubmed: 34583994 ]
- Uridine 5'-triphosphate (484 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Ctps1 tetramer (Complex from Homo sapiens)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Ctp synthase 1 (66 kDa, Protein from Homo sapiens)
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 7mh0
Q-score: 0.205
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2107968118 Pubmed: 34583994 ]
- Magnesium ion (24 Da, Ligand)
- Ctps1 tetramer (Complex from Homo sapiens)
- Ctp synthase 1 (66 kDa, Protein from Homo sapiens)
- Cytidine-5'-triphosphate (483 Da, Ligand)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mh1
Q-score: 0.536
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2107968118 Pubmed: 34583994 ]
- Magnesium ion (24 Da, Ligand)
- Ctps2 tetramer (Complex from Homo sapiens)
- Ctp synthase 2 (65 kDa, Protein from Homo sapiens)
- Cytidine-5'-triphosphate (483 Da, Ligand)
Human CTPS1 bound to inhibitor R80
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7mif
Q-score: 0.471
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ Pubmed: 34583994 DOI: doi:10.1073/pnas.2107968118 ]
- Ctps1 tetramer (Complex from Homo sapiens)
- Glutamine (146 Da, Ligand)
- N-(1-{2-[(cyclopropanesulfonyl)amino]-1,3-thiazol-4-yl}cyclopropyl)-5-(6-ethoxypyrazin-2-yl)pyridine-2-carboxamide (486 Da, Ligand)
- Ctp synthase 1 (66 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Uridine 5'-triphosphate (484 Da, Ligand)
Human CTPS1 bound to inhibitor T35
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7mig
Q-score: 0.538
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2107968118 Pubmed: 34583994 ]
- Uridine 5'-triphosphate (484 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- Ctps1 tetramer (Complex from Homo sapiens)
- 2-{2-[(cyclopropanesulfonyl)amino]-1,3-thiazol-4-yl}-2-methyl-n-{5-[6-(trifluoromethyl)pyrazin-2-yl]pyridin-2-yl}propanamide (512 Da, Ligand)
- Ctp synthase 1 (66 kDa, Protein from Homo sapiens)
Human CTPS2 bound to inhibitor R80
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7mih
Q-score: 0.55
Lynch EM, DiMattia MA, Albanese S, van Zundert GCP, Hansen JM, Quispe JD, Kennedy MA, Verras A, Borrelli K, Toms AV, Kaila N, Kreutter KD, McElwee JJ, Kollman JM
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2107968118 Pubmed: 34583994 ]
- Uridine 5'-triphosphate (484 Da, Ligand)
- Ctps2 tetramer (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Glutamine (146 Da, Ligand)
- N-(1-{2-[(cyclopropanesulfonyl)amino]-1,3-thiazol-4-yl}cyclopropyl)-5-(6-ethoxypyrazin-2-yl)pyridine-2-carboxamide (486 Da, Ligand)
- Ctp synthase 2 (65 kDa, Protein from Homo sapiens)