CryoEM structure of fospropofol-bound MRGPRX4-Gq complex
Chien DC, Limjunyawong N, Cao C, Meixiong J, Peng Q, Ho CY, Fay JF, Roth BL, Dong X
Sci Transl Med (2024) 16 pp. eadk8198-eadk8198 [ Pubmed: 38718132 DOI: doi:10.1126/scitranslmed.adk8198 ]
- Fospropofol-bound mrgprx4-gq complex (150 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4 (53 kDa, Protein from Homo sapiens)
- [2,6-di(propan-2-yl)phenoxy]methyl dihydrogen phosphate (288 Da, Ligand)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
The Cryo-EM structure of MPXV E5 apo conformation
Resolution: 3.32 Å
EM Method: Single-particle
Fitted PDBs: 8xj6
Q-score: 0.362
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Monkeypox virus (Virus from Monkeypox virus)
- Monkeypox virus e5 (90 kDa, Protein from Monkeypox virus)
- Amp phosphoramidate (426 Da, Ligand)
The Cryo-EM structure of MPXV E5 in complex with DNA
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 8xj7
Q-score: 0.541
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Monkeypox virus (Virus from Monkeypox virus)
- Magnesium ion (24 Da, Ligand)
- Dna (70-mer) (21 kDa, DNA from Monkeypox virus)
- Monkeypox virus e5 (90 kDa, Protein from Monkeypox virus)
The Cryo-EM structure of MPXV E5 C-terminal in complex with DNA
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8xj8
Q-score: 0.189
Zhang W, Liu Y, Yang M, Yang J, Shao Z, Gao Y, Jiang X, Cui R, Zhang Y, Zhao X, Shao Q, Cao C, Li H, Li L, Liu H, Gao H, Gan J
Cell Discov (2024) 10 pp. 67-67 [ DOI: doi:10.1038/s41421-024-00680-1 Pubmed: 38914567 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Monkeypox virus e5 (53 kDa, Protein from Monkeypox virus)
- Monkeypox virus (Virus from Monkeypox virus)
- Magnesium ion (24 Da, Ligand)
- Dna (70-mer) (21 kDa, DNA from Monkeypox virus)
CryoEM structure of Gi-coupled TAS2R14 with cholesterol and an intracellular tastant
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8vy7
Q-score: 0.52
Kim Y, Gumpper RH, Liu Y, Kocak DD, Xiong Y, Cao C, Deng Z, Krumm BE, Jain MK, Zhang S, Jin J, Roth BL
Nature (2024) 628 pp. 664-671 [ DOI: doi:10.1038/s41586-024-07253-y Pubmed: 38600377 ]
- Ternary complex of tas2r14 with gi heterotrimer (Complex from Homo sapiens)
- Taste receptor type 2 member 14 (70 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (42 kDa, Protein from Homo sapiens)
- 4-methyl-n-[(2m)-2-(1h-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine (321 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
CryoEM structure of Ggust-coupled TAS2R14 with cholesterol and an intracellular tastant
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 8vy9
Q-score: 0.479
Kim Y, Gumpper RH, Liu Y, Kocak DD, Xiong Y, Cao C, Deng Z, Krumm BE, Jain MK, Zhang S, Jin J, Roth BL
Nature (2024) 628 pp. 664-671 [ Pubmed: 38600377 DOI: doi:10.1038/s41586-024-07253-y ]
- Ternary complex of tas2r14 with ggust heterotrimer (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Taste receptor type 2 member 14,gpcr,taste receptor type 2 member 14 (72 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- 4-methyl-n-[(2m)-2-(1h-tetrazol-5-yl)phenyl]-6-(trifluoromethyl)pyrimidin-2-amine (321 Da, Ligand)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(t) subunit alpha-3 (40 kDa, Protein from Homo sapiens)
5-HT2B receptor bound to LSD obtained by cryo-electron microscopy (cryoEM)
Cao C, Barros-Álvarez X, Zhang S, Kim K, Dämgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Hüttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154-3167.e7 [ Pubmed: 36087581 DOI: 10.1016/j.neuron.2022.08.006 ]
Image sets:
Cao C, Barros-Álvarez X, Zhang S, Kim K, Dämgen MA, Panova O, Suomivuori CM, Fay JF, Zhong X, Krumm BE, Gumpper RH, Seven AB, Robertson MJ, Krogan NJ, Hüttenhain R, Nichols DE, Dror RO, Skiniotis G, Roth BL
Neuron (2022) 110 pp. 3154-3167.e7 [ Pubmed: 36087581 DOI: 10.1016/j.neuron.2022.08.006 ]
Image sets: