Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8fyh
Q-score: 0.4
Song J, Gooding AR, Hemphill WO, Love BD, Robertson A, Yao L, Zon LI, North TE, Kasinath V, Cech TR
Science (2023) 381 pp. 1331-1337 [ Pubmed: 37733873 DOI: doi:10.1126/science.adh0059 ]
- Polycomb protein eed (50 kDa, Protein from Homo sapiens)
- G4 rna (7 kDa, RNA from Homo sapiens)
- Protein jumonji isoform x3 (138 kDa, Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh2 (86 kDa, Protein from Homo sapiens)
- Polycomb protein suz12 (83 kDa, Protein from Homo sapiens)
- G4 rna-mediated dimer of polycomb repressive complex 2 (800 kDa, Complex from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histone-binding protein rbbp4 (47 kDa, Protein from Homo sapiens)
- Zinc finger protein aebp2 (54 kDa, Protein from Homo sapiens)
Body1 of G4 RNA-mediated PRC2 dimer from multibody refinement
Song J, Gooding AR, Hemphill WO, Love BD, Robertson A, Yao L, Zon LI, North TE, Kasinath V, Cech TR
Science (2023) 381 pp. 1331-1337 [ Pubmed: 37733873 DOI: doi:10.1126/science.adh0059 ]
- Zinc finger protein aebp2 (Protein from Homo sapiens)
- G4 rna-mediated dimer of polycomb repressive complex 2 (Complex from Homo sapiens)
- Polycomb protein suz12 (Protein from Homo sapiens)
- Polycomb protein eed (Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh2 (Protein from Homo sapiens)
- Protein jumonji isoform x3 (Protein from Homo sapiens)
- Histone-binding protein rbbp4 (Protein from Homo sapiens)
- G4 rna (RNA from Homo sapiens)
Body2 of G4 RNA-mediated PRC2 dimer from multibody refinement
Song J, Gooding AR, Hemphill WO, Love BD, Robertson A, Yao L, Zon LI, North TE, Kasinath V, Cech TR
Science (2023) 381 pp. 1331-1337 [ DOI: doi:10.1126/science.adh0059 Pubmed: 37733873 ]
- G4 rna-mediated dimer of polycomb repressive complex 2 (Complex from Homo sapiens)
- Protein jumonji isoform x3 (Protein from Homo sapiens)
- Polycomb protein eed (Protein from Homo sapiens)
- Polycomb protein suz12 (Protein from Homo sapiens)
- Zinc finger protein aebp2 (Protein from Homo sapiens)
- Histone-lysine n-methyltransferase ezh2 (Protein from Homo sapiens)
- Histone-binding protein rbbp4 (Protein from Homo sapiens)
The structure of human CST reveals a decameric assembly bound to telomeric DNA
Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR
Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]
- Homo sapiens cst complex subunit ctc1 (Protein from Homo sapiens)
- 3xtel (DNA from Homo sapiens)
- Decameric assembly of human cst complex with telomeric single-stranded dna (2 MDa, Complex from Homo sapiens)
- Homo sapiens cst complex subunit ten1 (Protein from Homo sapiens)
- Homo sapiens cst complex subunit stn1 (Protein from Homo sapiens)
Cryo-EM structure of CST bound to telomeric single-stranded DNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6w6w
Q-score: 0.536
Lim CJ, Barbour AT, Zaug AJ, Goodrich KJ, McKay AE, Wuttke DS, Cech TR
Science (2020) 368 pp. 1081-1085 [ DOI: doi:10.1126/science.aaz9649 Pubmed: 32499435 ]
- Dna (5'-d(p*tp*ap*gp*g)-3') (1 kDa, DNA from Homo sapiens)
- Cst complex subunit stn1 (43 kDa, Protein from Homo sapiens)
- Human cst complex (200 kDa, Complex from Homo sapiens)
- Cst complex subunit ten1 (17 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Cst complex subunit ctc1 (136 kDa, Protein from Homo sapiens)