Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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primary_citation_author:"Chen J" AND assembly_molecular_weight:{100000 TO 250000] AND author_orcid:"0000-0002-6829-8144" AND resolution:[4 TO 5}

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EMD-0412

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Cryo-EM structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and G protein

Release date: April 17, 2019
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6nbi
Q-score: 0.285

Zhao LH, Ma S, Sutkeviciute I, Shen DD, Zhou XE, de Waal PW, Li CY, Kang Y, Clark LJ, Jean-Alphonse FG, White AD, Yang D, Dai A, Cai X, Chen J, Li C, Jiang Y, Watanabe T, Gardella TJ, Melcher K, Wang MW, Vilardaga JP, Xu HE, Zhang Y

Science (2019) 364 pp. 148-153 [ DOI: doi:10.1126/science.aav7942 Pubmed: 30975883 ]

Sample:

Cholesterol (386 Da, Ligand)
Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
Long-acting parathyroid hormone analog (4 kDa, Protein from Homo sapiens)
Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
Gs protein alpha subunit (43 kDa, Protein from Bos taurus)
Nanobody-35 (13 kDa, Protein from synthetic construct)
Cryo-em structure of parathyroid hormone receptor type 1 in complex with a long-acting parathyroid hormone analog and g protein (Complex from Homo sapiens)
Parathyroid hormone/parathyroid hormone-related peptide receptor (54 kDa, Protein from Homo sapiens)

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