Cryo-EM structure of the 9-hydroxystearic acid bound GPR120-Gi complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8id3
Q-score: 0.522
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ DOI: doi:10.1126/science.add6220 Pubmed: 36862765 ]
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 9-hydroxyoctadecanoic acid (300 Da, Ligand)
- Cryo-em structure of the 9-hydroxystearic acid bound gpr120-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the linoleic acid bound GPR120-Gi complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8id4
Q-score: 0.547
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ DOI: doi:10.1126/science.add6220 Pubmed: 36862765 ]
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Linoleic acid (280 Da, Ligand)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cryo-em structure of the linoleic acid bound gpr120-gi complex (Complex from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the oleic acid bound GPR120-Gi complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8id6
Q-score: 0.532
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ Pubmed: 36862765 DOI: doi:10.1126/science.add6220 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Oleic acid (282 Da, Ligand)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of the oleic acid bound gpr120-gi complex (Complex from Homo sapiens)
Cryo-EM structure of the TUG891 bound GPR120-Gi complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8id8
Q-score: 0.524
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ DOI: doi:10.1126/science.add6220 Pubmed: 36862765 ]
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cryo-em structure of the tug891 bound gpr120-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- 3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid (364 Da, Ligand)
Cryo-EM structure of the eicosapentaenoic acid bound GPR120-Gi complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8id9
Q-score: 0.538
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ DOI: doi:10.1126/science.add6220 Pubmed: 36862765 ]
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- 5,8,11,14,17-eicosapentaenoic acid (302 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Cryo-em structure of the eicosapentaenoic acid bound gpr120-gi complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of the TUG891 bound GPR120-Giq complex
Resolution: 2.64 Å
EM Method: Single-particle
Fitted PDBs: 8g59
Q-score: 0.548
Mao C, Xiao P, Tao XN, Qin J, He QT, Zhang C, Guo SC, Du YQ, Chen LN, Shen DD, Yang ZS, Zhang HQ, Huang SM, He YH, Cheng J, Zhong YN, Shang P, Chen J, Zhang DL, Wang QL, Liu MX, Li GY, Guo Y, Xu HE, Wang C, Zhang C, Feng S, Yu X, Zhang Y, Sun JP
Science (2023) 380 pp. eadd6220-eadd6220 [ DOI: doi:10.1126/science.add6220 Pubmed: 36862765 ]
- Free fatty acid receptor 4 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(q) subunit alpha (40 kDa, Protein from Homo sapiens)
- Cryo-em structure of the tug891 bound gpr120-giq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- 3-{4-[(4-fluoro-4'-methyl[1,1'-biphenyl]-2-yl)methoxy]phenyl}propanoic acid (364 Da, Ligand)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8eig
Q-score: 0.506
Science (2022) 378 pp. 284-290 [ Pubmed: 36264792 DOI: doi:10.1126/science.ade2216 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (6p)-n-(1,3-dimethyl-1h-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1h-pyrazol-1-yl]-2-[(4s)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide (597 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- The complex of phosphorylated human delta f508 cystic fibrosis transmembrane conductance regulator (cftr) with elexacaftor (vx-445) and atp/mg (Complex from Homo sapiens)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8eio
Q-score: 0.579
Science (2022) 378 pp. 284-290 [ Pubmed: 36264792 DOI: doi:10.1126/science.ade2216 ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- The complex of phosphorylated human delta f508 cystic fibrosis transmembrane conductance regulator (cftr) with elexacaftor (vx-445), lumacaftor (vx-809) and atp/mg (168 kDa, Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- (6p)-n-(1,3-dimethyl-1h-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1h-pyrazol-1-yl]-2-[(4s)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide (597 Da, Ligand)
- Lumacaftor (452 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8eiq
Q-score: 0.583
Science (2022) 378 pp. 284-290 [ DOI: doi:10.1126/science.ade2216 Pubmed: 36264792 ]
- Tezacaftor (520 Da, Ligand)
- (6p)-n-(1,3-dimethyl-1h-pyrazole-4-sulfonyl)-6-[3-(3,3,3-trifluoro-2,2-dimethylpropoxy)-1h-pyrazol-1-yl]-2-[(4s)-2,2,4-trimethylpyrrolidin-1-yl]pyridine-3-carboxamide (597 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide (392 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- The complex of phosphorylated human delta f508 cystic fibrosis transmembrane conductance regulator (cftr) with trikafta [elexacaftor (vx-445), tezacaftor (vx-661), ivacaftor (vx-770)] and atp/mg (168 kDa, Complex from Homo sapiens)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- Dodecane (170 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
Dephosphorylated human delta F508 cystic fibrosis transmembrane conductance regulator (CFTR)
Resolution: 6.9 Å
EM Method: Single-particle
Fitted PDBs: 8ej1
Q-score: 0.334
Science (2022) 378 pp. 284-290 [ DOI: doi:10.1126/science.ade2216 Pubmed: 36264792 ]