Cryo-EM structure of succinate receptor bound to cis-epoxysuccinic acid coupling to Gi
Wang T, Tang W, Zhu X, Lv Z, Chen J, Li Y, Sun X, Lv H, Gu Q, Li F, Wang J
Cell Res (2024) [ Pubmed: 38744983 DOI: doi:10.1038/s41422-024-00968-7 ]
- Soluble cytochrome b562,succinate receptor 1,oplophorus-luciferin 2-monooxygenase catalytic subunit (75 kDa, Protein from Oplophorus gracilirostris)
- Gpcr complex of succinate receptor (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (30 kDa, Protein from Homo sapiens)
- (2r,3s)-oxirane-2,3-dicarboxylic acid (132 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
Cryo-EM structure of succinate receptor bound to succinate acid coupling MiniGsq
Wang T, Tang W, Zhu X, Lv Z, Chen J, Li Y, Sun X, Lv H, Gu Q, Li F, Wang J
Cell Res (2024) [ DOI: doi:10.1038/s41422-024-00968-7 Pubmed: 38744983 ]
- Soluble cytochrome b562,succinate receptor 1,oplophorus-luciferin 2-monooxygenase catalytic subunit (74 kDa, Protein from Oplophorus gracilirostris)
- Gpcr complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (41 kDa, Protein from Homo sapiens)
- Succinic acid (118 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
Cryo-EM structure of apo state human Cav3.2
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9ayg
Q-score: 0.537
Huang J, Fan X, Jin X, Lyu C, Guo Q, Liu T, Chen J, Davakan A, Lory P, Yan N
Cell Res (2024) 34 pp. 440-450 [ DOI: doi:10.1038/s41422-024-00959-8 Pubmed: 38605177 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (234 kDa, Protein from Homo sapiens)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (Complex from Homo sapiens)
Cryo-EM structure of human Cav3.2 with TTA-A2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9ayh
Q-score: 0.52
Huang J, Fan X, Jin X, Lyu C, Guo Q, Liu T, Chen J, Davakan A, Lory P, Yan N
Cell Res (2024) 34 pp. 440-450 [ Pubmed: 38605177 DOI: doi:10.1038/s41422-024-00959-8 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 2-(4-cyclopropylphenyl)-n-{(1r)-1-[5-(2,2,2-trifluoroethoxy)pyridin-2-yl]ethyl}acetamide (378 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (234 kDa, Protein from Homo sapiens)
- Voltage-dependent t-type calcium channel subunit alpha-1h (Complex from Homo sapiens)
- Calcium ion (40 Da, Ligand)
Cryo-EM structure of human Cav3.2 with TTA-P2
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9ayj
Q-score: 0.484
Huang J, Fan X, Jin X, Lyu C, Guo Q, Liu T, Chen J, Davakan A, Lory P, Yan N
Cell Res (2024) 34 pp. 440-450 [ DOI: doi:10.1038/s41422-024-00959-8 Pubmed: 38605177 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 3,5-dichloro-n-[(1-{[(4s)-2,2-dimethyloxan-4-yl]methyl}-4-fluoropiperidin-4-yl)methyl]benzamide (431 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (234 kDa, Protein from Homo sapiens)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (Complex from Homo sapiens)
Cryo-EM structure of human Cav3.2 with ML218
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9ayk
Q-score: 0.519
Huang J, Fan X, Jin X, Lyu C, Guo Q, Liu T, Chen J, Davakan A, Lory P, Yan N
Cell Res (2024) 34 pp. 440-450 [ DOI: doi:10.1038/s41422-024-00959-8 Pubmed: 38605177 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (234 kDa, Protein from Homo sapiens)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (Complex from Homo sapiens)
- 3,5-dichloro-n-{[(1r,5s,6r)-3-(3,3-dimethylbutyl)-3-azabicyclo[3.1.0]hexan-6-yl]methyl}benzamide (369 Da, Ligand)
Cryo-EM structure of human Cav3.2 with ACT-709478
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 9ayl
Q-score: 0.531
Huang J, Fan X, Jin X, Lyu C, Guo Q, Liu T, Chen J, Davakan A, Lory P, Yan N
Cell Res (2024) 34 pp. 440-450 [ DOI: doi:10.1038/s41422-024-00959-8 Pubmed: 38605177 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- 1-o-octadecyl-sn-glycero-3-phosphocholine (510 Da, Ligand)
- N-{1-[(5-cyanopyridin-2-yl)methyl]-1h-pyrazol-3-yl}-2-{4-[1-(trifluoromethyl)cyclopropyl]phenyl}acetamide (425 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (234 kDa, Protein from Homo sapiens)
- [(2~{r})-3-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-2-pentadecanoyloxy-propyl] pentadecanoate (663 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Voltage-dependent t-type calcium channel subunit alpha-1h (Complex from Homo sapiens)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7sv7
Q-score: 0.463
Cell (2022) 185 pp. 158-168.e11 [ Pubmed: 34995514 DOI: doi:10.1016/j.cell.2021.12.009 ]
- Tetradecane (198 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- Dodecane (170 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Tezacaftor (520 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Nonane (128 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- The complex of phosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) with atp/mg and tezacaftor (vx-661) (Complex from Homo sapiens)
- Heptadecane (240 Da, Ligand)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7svd
Q-score: 0.585
Cell (2022) 185 pp. 158-168.e11 [ DOI: doi:10.1016/j.cell.2021.12.009 Pubmed: 34995514 ]
- The complex of phosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) with atp/mg and lumacaftor (vx-809) (Complex from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)
- Dodecane (170 Da, Ligand)
- Water (18 Da, Ligand)
- Unknown atom or ion (103 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Lumacaftor (452 Da, Ligand)
- Decane (142 Da, Ligand)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7svr
Q-score: 0.42
Cell (2022) 185 pp. 158-168.e11 [ DOI: doi:10.1016/j.cell.2021.12.009 Pubmed: 34995514 ]
- The complex of dephosphorylated human cystic fibrosis transmembrane conductance regulator (cftr) and lumacaftor (vx-809) (Complex from Homo sapiens)
- Lumacaftor (452 Da, Ligand)
- Cystic fibrosis transmembrane conductance regulator (168 kDa, Protein from Homo sapiens)
- Cystic fibrosis transmembrane conductance regulator (1 kDa, Protein from Homo sapiens)