Electron Microscopy Data Bank

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Structure of Mce1-LucB complex from Mycobacterium smegmatis (Map1)

Release date: Feb. 22, 2023
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8fed
Q-score: 0.524

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

Unknown ligand (Ligand)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) bound to accessory factor lucb (Complex from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Transmembrane protein (20 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-29024

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Structure of Mce1 transporter from Mycobacterium smegmatis in the absence of LucB (Map2)

Release date: Feb. 22, 2023
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8fee
Q-score: 0.502

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Unknown ligand (Ligand)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-29025

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Structure of Mce1 transporter from Mycobacterium smegmatis (Map0)

Release date: Feb. 22, 2023
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8fef
Q-score: 0.564

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

Unknown ligand (Ligand)
Virulence factor mce family protein (58 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Conserved hypothetical integral membrane protein yrbe1a (27 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce1 lipid transporter composed of mce1 mce proteins (mce1abcdef) and an abc transporter (yrbe1a-b, 2 copies of mceg) (Complex from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (43 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (42 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc transporter, atp-binding protein,green fluorescent protein chimera (71 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Virulence factor mce family protein (37 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1c (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Mce-family protein mce1f (54 kDa, Protein from Mycolicibacterium smegmatis MC2 155)
Abc-transporter integral membrane protein (30 kDa, Protein from Mycolicibacterium smegmatis MC2 155)

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EMD-29228

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Local refinement of MceG in Msmeg Mce1 transporter (Map0a)

Release date: Feb. 15, 2023
Resolution: 2.91 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29229

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Local refinement of YrbE and MCE ring in Msmeg Mce1 transporter (Map0b)

Release date: Feb. 15, 2023
Resolution: 2.73 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29230

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Local refinement of MCE ring and needle in Msmeg Mce1 transporter (Map0c)

Release date: Feb. 15, 2023
Resolution: 2.75 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29231

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Local refinement of MCE needle and portal in Msmeg Mce1 transporter (Map0d)

Release date: Feb. 15, 2023
Resolution: 3.0 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29232

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Raw, consensus refinement of Msmeg Mce1 transporter (Map0e)

Release date: Feb. 15, 2023
Resolution: 2.92 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29233

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Local refinement of MceG in Msmeg Mce1-LucB complex (Map1a)

Release date: Feb. 15, 2023
Resolution: 3.05 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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EMD-29234

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Local refinement of YrbE, MCE ring, LucB in Msmeg Mce1-LucB complex (Map1b)

Release date: Feb. 15, 2023
Resolution: 2.89 Å
EM Method: Single-particle

Chen J, Fruhauf A, Fan C, Ponce J, Ueberheide B, Bhabha G, Ekiert DC

Nature (2023) 620 pp. 445-452 [ DOI: doi:10.1038/s41586-023-06366-0 Pubmed: 37495693 ]

Sample:

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