CryoEM of Mycobacterium tuberculosis WT RNAP holoenzyme/RbpA bound to the Fdx and upstream fork DNA
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
Elife (2018) 7 [ Pubmed: 29480804 DOI: 10.7554/elife.34823 ]
Image sets:
Mtb RNAP Holo/RbpA/Fidaxomicin/upstream fork DNA
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 6bzo
Q-score: 0.489
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
eLife (2018) 7 [ Pubmed: 29480804 DOI: doi:10.7554/eLife.34823 ]
- Dna (32-mer) (9 kDa, DNA from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Fidaxomicin (1 kDa, Ligand)
- Rna polymerase sigma factor siga (58 kDa, Protein from Mycobacterium tuberculosis)
- Complex of mtb rnap with an antibiotic called fidaxomicin (Complex from Mycobacterium tuberculosis)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (148 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna (26-mer) (7 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)
Mtb RNAP Holo/RbpA/double fork DNA -closed clamp
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 6c04
Q-score: 0.515
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
eLife (2018) 7 [ Pubmed: 29480804 DOI: doi:10.7554/eLife.34823 ]
- Dna (31-mer) (9 kDa, DNA from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (148 kDa, Protein from Mycobacterium tuberculosis)
- Rna polymerase sigma factor siga (58 kDa, Protein from Mycobacterium tuberculosis)
- Mtb rnap holo/rbpa/double fork dna (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna (26-mer) (7 kDa, DNA from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP Holo/RbpA in relaxed state
Resolution: 5.15 Å
EM Method: Single-particle
Fitted PDBs: 6c05
Q-score: 0.271
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
eLife (2018) 7 [ Pubmed: 29480804 DOI: doi:10.7554/eLife.34823 ]
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (148 kDa, Protein from Mycobacterium tuberculosis)
- Rna polymerase sigma factor siga (58 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap holo with rbpa (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP Holo/RbpA/Fidaxomicin
Resolution: 5.15 Å
EM Method: Single-particle
Fitted PDBs: 6c06
Q-score: 0.287
Boyaci H, Chen J, Lilic M, Palka M, Mooney RA, Landick R, Darst SA, Campbell EA
eLife (2018) 7 [ Pubmed: 29480804 DOI: doi:10.7554/eLife.34823 ]
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (58 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap holo with rbpa and fidaxomicin (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Mycobacterium tuberculosis)
- Fidaxomicin (1 kDa, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (148 kDa, Protein from Mycobacterium tuberculosis)
- Rna polymerase-binding protein rbpa (12 kDa, Protein from Mycobacterium tuberculosis)