Yeast C, Ci, C*, and P complex spliceosomes
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452 [ Pubmed: 33705709 DOI: 10.1016/j.molcel.2021.02.021 ]
Image sets:
- Unaligned movies of C, C*, and P-complex spliceosomes with dominant-negative Prp22 mutation K512A, purified with Slu7 (Dataset 6)
- Unaligned movies of C-complex spliceosome with 3' splice site AG to AC mutation (Dataset 1)
- Unaligned movies of C and C*-complex spliceosomes with 3' splice site AG to AdG mutation (Dataset 3)
- Various signal subtractions for C- and P-complex spliceosomes
- Selected P-complex particles after polishing
- Unaligned movies of C-complex spliceosomes with cold-sensitive prp16-302 mutation, purified with Cwc25 and incubated with ATP and Mg (Dataset 5)
- Selected C-complex particles after polishing
- Aligned movies of C-complex spliceosomes with cold-sensitive prp16-302 mutation, purified with Cwc25 (Dataset 4)
- Unaligned movies of C and C*-complex spliceosomes with 3' splice site AG to AdG mutation (Dataset 2)
- Unaligned movies of P-complex spliceosomes with dominant-negative Prp22 mutation K512A, treated with anti-3'exon RNaseH oligo, purified in presence of Mg (Dataset 9)
Yeast C complex spliceosome, focused refinement on U2 snRNP
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
Yeast C complex spliceosome at 2.8 Angstrom resolution with Prp18/Slu7 bound, composite map
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7b9v
Q-score: 0.378
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439- [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
- Clf1 isoform 1 (82 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing helicase brr2 (246 kDa, Protein from Baker's yeast)
- 5' exon of ubc4 mrna (15 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Syf1 isoform 1 (100 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor cwc25 (20 kDa, Protein from Baker's yeast)
- Bj4_g0054360.mrna.1.cds.1 (50 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- D-chiro inositol hexakisphosphate (660 Da, Ligand)
- Snt309 isoform 1 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (27 kDa, Protein from Baker's yeast)
- Potassium ion (39 Da, Ligand)
- Branched intron and 3' exon of ubc4 pre-mrna (30 kDa, RNA from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- Cwc22 isoform 1 (67 kDa, Protein from Baker's yeast)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Baker's yeast)
- Ntc20 isoform 1 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Splicing factor yju2 (33 kDa, Protein from Baker's yeast)
- Cdc40 isoform 1 (52 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Y55_g0042700.mrna.1.cds.1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slu7 (44 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 18 (28 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-splicing factor atp-dependent rna helicase prp16 (121 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Unassigned structure (20 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Bud31 isoform 1 (18 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Yeast c complex spliceosome with prp18/slu7 bound (2 MDa, Complex from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
Yeast C complex spliceosome, focused refinement on NTC
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
Yeast C complex spliceosome, focused refinement on Brr2/Prp8-Jab1 and Prp16
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
Yeast C complex spliceosome, focused refinement on just Brr2/Prp8-Jab1
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
Yeast C complex spliceosome, overall map after focused classification on Prp17
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ Pubmed: 33705709 DOI: doi:10.1016/j.molcel.2021.02.021 ]
Yeast C complex spliceosome, overall map after focused classification on Slu7 zinc knuckle
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ DOI: doi:10.1016/j.molcel.2021.02.021 Pubmed: 33705709 ]
Yeast C complex spliceosome, overall map after focused classification on Prp18
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ DOI: doi:10.1016/j.molcel.2021.02.021 Pubmed: 33705709 ]
Wilkinson ME, Fica SM, Galej WP, Nagai K
Mol Cell (2021) 81 pp. 1439-1452.e9 [ DOI: doi:10.1016/j.molcel.2021.02.021 Pubmed: 33705709 ]