Electron Microscopy Data Bank

EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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primary_citation_author:"Florez-Ariza A"

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EMD-0340

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EM structure of the DNA wrapping in bacterial open transcription initiation complex

Release date: May 27, 2020
Resolution: 17.0 Å
EM Method: Single-particle
Fitted PDBs: 6n4c
Q-score: 0.019

Sosa R, Florez-Ariza A, Diaz-Celis C, Onoa B, Cassago A, de Oliveira PSL, Portugal RV, Guerra DG, Bustamante C

bioRxiv (2020) [ DOI: doi:10.1101/2020.05.13.070375 ]

Sample:

Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
Rna polymerase sigma factor rpod (64 kDa, Protein from Escherichia coli K-12)
Dna (94-mer) (29 kDa, DNA from Enterobacteria phage lambda)
Dna-directed rna polymerase subunit alpha (34 kDa, Protein from Escherichia coli K-12)
E. coli rnap-dna wrapped open complex (500 kDa, Complex from Escherichia coli BL21)
Dna (94-mer) (28 kDa, DNA from Enterobacteria phage lambda)
Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
Dna-directed rna polymerase subunit beta' (150 kDa, Protein from Escherichia coli K-12)

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