Mycobacterium tuberculosis RNAP paused elongation complex with NusG transcription factor
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8e74
Q-score: 0.543
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap paused elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
Resolution: 3.71 Å
EM Method: Single-particle
Fitted PDBs: 8e79
Q-score: 0.449
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Mycobacterium tuberculosis rnap paused elongation complex with escherchia coli nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Magnesium ion (24 Da, Ligand)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
Mycobacterium tuberculosis RNAP elongation complex with NusG transcription factor
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8e82
Q-score: 0.447
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap elongation complex with nusg transcription factor (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Transcription termination/antitermination protein nusg (25 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
Mycobacterium tuberculosis RNAP paused elongation complex
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8e8m
Q-score: 0.496
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Mycobacterium tuberculosis rnap paused elongation complex (Complex from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (54-mer) (16 kDa, DNA from synthetic construct)
- Rna (42-mer) (13 kDa, RNA from synthetic construct)
Mycobacterium tuberculosis RNAP elongation complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8e95
Q-score: 0.497
Delbeau M, Omollo EO, Froom R, Koh S, Mooney RA, Lilic M, Brewer JJ, Rock J, Darst SA, Campbell EA, Landick R
Mol Cell (2023) 83 pp. 1474-1488.e8 [ Pubmed: 37116494 DOI: doi:10.1016/j.molcel.2023.04.007 ]
- Mycobacterium tuberculosis rnap elongation complex (Complex from Mycobacterium tuberculosis)
- Rna (5'-r(p*cp*gp*gp*ap*gp*ap*gp*gp*up*a)-3') (6 kDa, RNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (11 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit alpha (37 kDa, Protein from Mycobacterium tuberculosis)
- Dna-directed rna polymerase subunit beta' (147 kDa, Protein from Mycobacterium tuberculosis)
- Dna (25-mer) (16 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta (129 kDa, Protein from Mycobacterium tuberculosis)
- Zinc ion (65 Da, Ligand)
- Dna (33-mer) (16 kDa, DNA from synthetic construct)
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8dy7
Q-score: 0.482
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ DOI: doi:10.1073/pnas.2220785120 Pubmed: 36888660 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Dna (78-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Dna (71-mer) (30 kDa, DNA from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae)
- Probable cell division protein whia (37 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Streptomyces venezuelae rnap transcription open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8dy9
Q-score: 0.492
Lilic M, Holmes NA, Bush MJ, Marti AK, Widdick DA, Findlay KC, Choi YJ, Froom R, Koh S, Buttner MJ, Campbell EA
PNAS (2023) 120 pp. e2220785120-e2220785120 [ Pubmed: 36888660 DOI: doi:10.1073/pnas.2220785120 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Magnesium ion (24 Da, Ligand)
- Rna polymerase sigma factor siga (56 kDa, Protein from Streptomyces venezuelae)
- Rna polymerase-binding protein rbpa (14 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta' (144 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Streptomyces venezuelae ATCC 10712)
- Probable cell division protein whia (34 kDa, Protein from Streptomyces venezuelae)
- Zinc ion (65 Da, Ligand)
- Dna (38-mer) (15 kDa, DNA from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit omega (9 kDa, Protein from Streptomyces venezuelae)
- Transcriptional regulator whib (10 kDa, Protein from Streptomyces venezuelae)
- Dna-directed rna polymerase subunit beta (130 kDa, Protein from Streptomyces venezuelae)
- Streptomyces venezuelae rnap unconstrained open promoter complex with whia and whib transcription factors (Complex from Streptomyces venezuelae)
Oligo nucleosome fraction from metaphase chromosome in Xenopus egg extract (lot1)
Arimura Y, Shih RM, Froom R, Funabiki H
Mol Cell (2021) [ Pubmed: 34478647 DOI: 10.1016/j.molcel.2021.08.010 ]
Image sets:
Oligo nucleosome fraction from interphase chromosome in Xenopus egg extract lot 1
Arimura Y, Shih RM, Froom R, Funabiki H
Mol Cell (2021) [ Pubmed: 34478647 DOI: 10.1016/j.molcel.2021.08.010 ]
Image sets:
Mono nucleosome fraction from interphase chromosome in Xenopus egg extract lot1
Arimura Y, Shih RM, Froom R, Funabiki H
Mol Cell (2021) [ Pubmed: 34478647 DOI: 10.1016/j.molcel.2021.08.010 ]
Image sets: