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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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primary_citation_author:"Gao Y" AND resolution:[3 TO 4} AND author_orcid:"0000-0001-9223-774x" AND natural_source_ncbi_code:"32630" AND author_name:"Rao Z" AND author_name:"Rao ZH" AND author_name:"Guddat LW"

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EMD-34318

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A mechanism for SARS-CoV-2 RNA capping and its inhibition by nucleotide analogue inhibitors

Release date: Nov. 30, 2022
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8gwo
Q-score: 0.362

Yan L, Huang Y, Ge J, Liu Z, Lu P, Huang B, Gao S, Wang J, Tan L, Ye S, Yu F, Lan W, Xu S, Zhou F, Shi L, Guddat LW, Gao Y, Rao Z, Lou Z

Cell (2022) 185 pp. 4347-4360.e17 [ DOI: doi:10.1016/j.cell.2022.09.037 Pubmed: 36335936 ]

Sample:

Helicase (66 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
E-rtc_ump-nsp9_gmppnp (Complex from Severe acute respiratory syndrome coronavirus 2)
Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Rna (25-mer) (8 kDa, RNA from synthetic construct)
Rna (Complex)
Replicase polyprotein (Complex)
Non-structural protein 9 (12 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Zinc ion (65 Da, Ligand)
Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
Uridine-5'-monophosphate (324 Da, Ligand)
Template (10 kDa, RNA from synthetic construct)

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