Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase in Apo state conformation I
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8foc
Q-score: 0.407
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in Apo state conformation II
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8fod
Q-score: 0.357
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex bound to a template DNA
Resolution: 5.6 Å
EM Method: Single-particle
Fitted PDBs: 8foe
Q-score: 0.212
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the RNA synthesis state
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8foh
Q-score: 0.358
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Primer rna (2 kDa, RNA from synthetic construct)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (3 kDa, DNA from synthetic construct)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 8foj
Q-score: 0.336
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ Pubmed: 37344454 DOI: doi:10.1038/s41467-023-39441-1 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Primer rna (3 kDa, RNA from synthetic construct)
- Template dna (3 kDa, DNA from synthetic construct)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of S. cerevisiae DNA polymerase alpha-primase complex in the DNA elongation state
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8fok
Q-score: 0.454
Yuan Z, Georgescu R, Li H, O'Donnell ME
Nat Commun (2023) 14 pp. 3697-3697 [ DOI: doi:10.1038/s41467-023-39441-1 Pubmed: 37344454 ]
- Iron/sulfur cluster (351 Da, Ligand)
- Template dna (6 kDa, DNA from synthetic construct)
- Dna polymerase alpha/primase complex (Complex from Saccharomyces cerevisiae)
- Dna primase (47 kDa, Protein from Saccharomyces cerevisiae)
- Rna-dna chimeric primer (4 kDa, RNA from synthetic construct)
- Dna primase large subunit (62 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase (167 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase alpha subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
SV40 T-Antigen helicase hexamer
Langston LD, Yuan Z, Georgescu R, Li H, O'Donnell ME
PNAS (2022) 119 pp. e2216240119-e2216240119 [ Pubmed: 36442086 DOI: doi:10.1073/pnas.2216240119 ]
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7tfh
Q-score: 0.469
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ Pubmed: 35829698 DOI: doi:10.7554/eLife.77469 ]
- Rfc-pcna-dna1-dna2 (Complex)
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Proteins (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Primer strand (6 kDa, DNA from synthetic construct)
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 7tfi
Q-score: 0.444
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Proteins (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Rfc-pcna-dna1-dna2 (Complex)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7tfj
Q-score: 0.458
Zheng F, Georgescu R, Yao NY, Li H, O'Donnell ME
eLife (2022) 11 [ DOI: doi:10.7554/eLife.77469 Pubmed: 35829698 ]
- Dsdna (Complex from synthetic construct)
- Replication factor c subunit 1 (95 kDa, Protein from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Replication factor c subunit 4 (36 kDa, Protein from Saccharomyces cerevisiae)
- Template strand (12 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proliferating cell nuclear antigen (29 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 2 (39 kDa, Protein from Saccharomyces cerevisiae)
- Primer strand (6 kDa, DNA from synthetic construct)
- Proteins (Complex from Saccharomyces cerevisiae)
- Replication factor c subunit 5 (39 kDa, Protein from Saccharomyces cerevisiae)
- Replication factor c subunit 3 (38 kDa, Protein from Saccharomyces cerevisiae)
- Rfc-pcna-dna1 (Complex)