Consensus refinement of the h12-LOX in a dimeric form
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The local refinement map of a "closed" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of an "open" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The consensus map of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of a single subunit of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The structure of h12-LOX in monomeric form
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8ghb
Q-score: 0.533
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Monomeric human 12-lipoxygenase (75 kDa, Complex from Homo sapiens)
The structure of h12-LOX in dimeric form
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8ghc
Q-score: 0.615
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Dimeric human 12-lipoxygenase (150 kDa, Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
The structure of h12-LOX in hexameric form bound to inhibitor ML355 and arachidonic acid
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8ghd
Q-score: 0.654
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
- Fe (ii) ion (55 Da, Ligand)
- N-(1,3-benzothiazol-2-yl)-4-{[(2-hydroxy-3-methoxyphenyl)methyl]amino}benzene-1-sulfonamide (441 Da, Ligand)
- Hexameric human 12-lipoxygenase in complex with inhibitor ml355 and arachidonic acid (450 kDa, Complex from Homo sapiens)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Arachidonic acid (304 Da, Ligand)
The structure of h12-LOX in tetrameric form bound to endogenous inhibitor oleoyl-CoA
Resolution: 2.05 Å
EM Method: Single-particle
Fitted PDBs: 8ghe
Q-score: 0.624
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Water (18 Da, Ligand)
- S-{(3r,5r,9r)-1-[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} (9z)-octadec-9-enethioate (non-preferred name) (1 kDa, Ligand)
- Tetrameric human 12-lipoxygenase bound to endogenous inhibitor oleoyl-coa (300 kDa, Complex from Homo sapiens)
SARS-CoV-2 Spike bound to Fab PDI 210
Wheatley AK, Pymm P, Esterbauer R, Dietrich MH, Lee WS, Drew D, Kelly HG, Chan LJ, Mordant FL, Black KA, Adair A, Tan HX, Juno JA, Wragg KM, Amarasena T, Lopez E, Selva KJ, Haycroft ER, Cooney JP, Venugopal H, Tan LL, O Neill MT, Allison CC, Cromer D, Davenport MP, Bowen RA, Chung AW, Pellegrini M, Liddament MT, Glukhova A, Subbarao K, Kent SJ, Tham WH
Cell Rep (2021) 37 pp. 109822-109822 [ Pubmed: 34610292 DOI: doi:10.1016/j.celrep.2021.109822 ]