Human M4 muscarinic acetylcholine receptor complex with Gi1 and xanomeline
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 8fx5
Q-score: 0.595
Burger WAC, Pham V, Vuckovic Z, Powers AS, Mobbs JI, Laloudakis Y, Glukhova A, Wootten D, Tobin AB, Sexton PM, Paul SM, Felder CC, Danev R, Dror RO, Christopoulos A, Valant C, Thal DM
Nat Commun (2023) 14 pp. 5440-5440 [ DOI: doi:10.1038/s41467-023-41199-5 Pubmed: 37673901 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Mus musculus)
- Xanomeline (281 Da, Ligand)
- M4 machr bound to agonist xanomeline in complex with dominant negative galpha-i1, gbeta1, ggamma2, and scfv16 (Complex)
- Muscarinic acetylcholine receptor m4 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Antibody fragment scfv16 (26 kDa, Protein from Homo sapiens)
Consensus refinement of the h12-LOX in a dimeric form
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The local refinement map of a "closed" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of an "open" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The consensus map of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of a single subunit of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The structure of h12-LOX in monomeric form
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8ghb
Q-score: 0.533
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Monomeric human 12-lipoxygenase (75 kDa, Complex from Homo sapiens)
The structure of h12-LOX in dimeric form
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8ghc
Q-score: 0.615
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Dimeric human 12-lipoxygenase (150 kDa, Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
The structure of h12-LOX in hexameric form bound to inhibitor ML355 and arachidonic acid
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8ghd
Q-score: 0.654
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
- Fe (ii) ion (55 Da, Ligand)
- N-(1,3-benzothiazol-2-yl)-4-{[(2-hydroxy-3-methoxyphenyl)methyl]amino}benzene-1-sulfonamide (441 Da, Ligand)
- Hexameric human 12-lipoxygenase in complex with inhibitor ml355 and arachidonic acid (450 kDa, Complex from Homo sapiens)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Arachidonic acid (304 Da, Ligand)
The structure of h12-LOX in tetrameric form bound to endogenous inhibitor oleoyl-CoA
Resolution: 2.05 Å
EM Method: Single-particle
Fitted PDBs: 8ghe
Q-score: 0.624
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Water (18 Da, Ligand)
- S-{(3r,5r,9r)-1-[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} (9z)-octadec-9-enethioate (non-preferred name) (1 kDa, Ligand)
- Tetrameric human 12-lipoxygenase bound to endogenous inhibitor oleoyl-coa (300 kDa, Complex from Homo sapiens)