Structure of KefC Asp156Asn variant
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 9emb
Q-score: 0.524
To Be Published
- Homodimer of kefc asp156asn variant (Complex from Escherichia coli)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Glutathione-regulated potassium-efflux system protein kefc (61 kDa, Protein from Escherichia coli)
Cryo-EM structure of Sodium proton exchanger NhaA with bound cardiolipin
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 8ps0
Q-score: 0.465
Kokane S, Meier P, Gulati A, Matsuoka R, Pipatpolkai T, Delemotte L, Drew D
To Be Published
- Cardiolipin (1 kDa, Ligand)
- Nhaa dimer (Complex from Escherichia coli)
- Na(+)/h(+) antiporter nhaa (42 kDa, Protein from Escherichia coli)
Structure of the K+/H+ exchanger KefC with GSH
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 8by2
Q-score: 0.507
Gulati A, Kokane S, Perez-Boerema A, Alleva C, Meier PF, Matsuoka R, Drew D
Nat Commun (2024) 15 pp. 4751-4751 [ Pubmed: 38834573 DOI: doi:10.1038/s41467-024-49082-7 ]
- Adenosine monophosphate (347 Da, Ligand)
- Glutathione-regulated potassium-efflux system protein kefc (61 kDa, Protein from Escherichia coli)
- Glutathione (307 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Kefc protein dimer with gsh (Complex from Escherichia coli)
- Potassium ion (39 Da, Ligand)
Structure of the K/H exchanger KefC
Resolution: 3.16 Å
EM Method: Single-particle
Fitted PDBs: 8bxg
Q-score: 0.519
Gulati A, Kokane S, Perez-Boerema A, Alleva C, Meier PF, Matsuoka R, Drew D
Nat Commun (2024) 15 pp. 4751-4751 [ Pubmed: 38834573 DOI: doi:10.1038/s41467-024-49082-7 ]
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Glutathione-regulated potassium-efflux system protein kefc (61 kDa, Protein from Escherichia coli)
- Adenosine monophosphate (347 Da, Ligand)
- Kefc protein dimer (Complex from Escherichia coli)
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 8thi
Q-score: 0.411
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ Pubmed: 38349818 DOI: doi:10.7554/eLife.92307 ]
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
Resolution: 2.99 Å
EM Method: Single-particle
Fitted PDBs: 8thj
Q-score: 0.52
Currie MJ, Davies JS, Scalise M, Gulati A, Wright JD, Newton-Vesty MC, Abeysekera GS, Subramanian R, Wahlgren WY, Friemann R, Allison JR, Mace PD, Griffin MDW, Demeler B, Wakatsuki S, Drew D, Indiveri C, Dobson RCJ, North RA
eLife (2024) 12 [ DOI: doi:10.7554/eLife.92307 Pubmed: 38349818 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- Sialic acid trap transporter permease protein siat (72 kDa, Protein from Haemophilus influenzae Rd KW20)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Hisiaqm transporter protein solubilised in amphipol a8-35 (Complex from Haemophilus influenzae Rd KW20)
- Sodium ion (22 Da, Ligand)
Cryo-EM structure of Strongylocentrotus purpuratus sperm-specific Na+/H+ exchanger SLC9C1 in GDN
Resolution: 3.22 Å
EM Method: Single-particle
Fitted PDBs: 8otq
Q-score: 0.419
Yeo H, Mehta V, Gulati A, Drew D
Nature (2023) 623 pp. 193-201 [ DOI: doi:10.1038/s41586-023-06518-2 Pubmed: 37880360 ]
- Sperm-specific sodium proton exchanger (147 kDa, Protein from Strongylocentrotus purpuratus)
- 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (758 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Homo-dimer of slc9c1 transporter (294 kDa, Complex from Strongylocentrotus purpuratus)
Cryo-EM structure of Strongylocentrotus purpuratus SLC9C1 in presence of cAMP
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 8otw
Q-score: 0.382
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8otx
Q-score: 0.457
Yeo H, Mehta V, Gulati A, Drew D
Nature (2023) 623 pp. 193-201 [ DOI: doi:10.1038/s41586-023-06518-2 Pubmed: 37880360 ]
- Water (18 Da, Ligand)
- Sperm-specific sodium proton exchanger (147 kDa, Protein from Strongylocentrotus purpuratus)
- Homo-dimer of slc9c1 transporter (294 kDa, Complex from Strongylocentrotus purpuratus)
- 1,2-diacyl-glycerol-3-sn-phosphate (704 Da, Ligand)
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8b01
Q-score: 0.539
Davies JS, Currie MJ, North RA, Scalise M, Wright JD, Copping JM, Remus DM, Gulati A, Morado DR, Jamieson SA, Newton-Vesty MC, Abeysekera GS, Ramaswamy S, Friemann R, Wakatsuki S, Allison JR, Indiveri C, Drew D, Mace PD, Dobson RCJ
Nat Commun (2023) 14 pp. 1120-1120 [ DOI: doi:10.1038/s41467-023-36590-1 Pubmed: 36849793 ]
- Phosphatidylethanolamine (734 Da, Ligand)
- Siaqm with megabody (Complex)
- Siaqm (Complex from Photobacterium profundum)
- Docosane (310 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Megabody c7hopq (54 kDa, Protein from synthetic construct)
- Megabody (Complex from Helicobacter pylori)
- Hexane (86 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, large permease component (45 kDa, Protein from Photobacterium profundum SS9)
- Tridecane (184 Da, Ligand)
- Putative trap-type c4-dicarboxylate transport system, small permease component (19 kDa, Protein from Photobacterium profundum SS9)
- N-octane (114 Da, Ligand)
- Decane (142 Da, Ligand)