Consensus refinement of the h12-LOX in a dimeric form
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The local refinement map of a "closed" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of an "open" subunit of a 12-LOX dimer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The consensus map of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
The local refinement map of a single subunit of a 12-LOX hexamer
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
The structure of h12-LOX in monomeric form
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 8ghb
Q-score: 0.533
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Monomeric human 12-lipoxygenase (75 kDa, Complex from Homo sapiens)
The structure of h12-LOX in dimeric form
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8ghc
Q-score: 0.615
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Dimeric human 12-lipoxygenase (150 kDa, Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
The structure of h12-LOX in hexameric form bound to inhibitor ML355 and arachidonic acid
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8ghd
Q-score: 0.654
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ DOI: doi:10.1182/blood.2023020441 Pubmed: 37506345 ]
- Fe (ii) ion (55 Da, Ligand)
- N-(1,3-benzothiazol-2-yl)-4-{[(2-hydroxy-3-methoxyphenyl)methyl]amino}benzene-1-sulfonamide (441 Da, Ligand)
- Hexameric human 12-lipoxygenase in complex with inhibitor ml355 and arachidonic acid (450 kDa, Complex from Homo sapiens)
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Arachidonic acid (304 Da, Ligand)
The structure of h12-LOX in tetrameric form bound to endogenous inhibitor oleoyl-CoA
Resolution: 2.05 Å
EM Method: Single-particle
Fitted PDBs: 8ghe
Q-score: 0.624
Mobbs JI, Black KA, Tran M, Burger WAC, Venugopal H, Holman TR, Holinstat M, Thal DM, Glukhova A
Blood (2023) 142 pp. 1233-1242 [ Pubmed: 37506345 DOI: doi:10.1182/blood.2023020441 ]
- Polyunsaturated fatty acid lipoxygenase alox12 (76 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Water (18 Da, Ligand)
- S-{(3r,5r,9r)-1-[(2r,3s,4r,5r)-5-(6-amino-9h-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9-trihydroxy-8,8-dimethyl-3,5-dioxido-10,14-dioxo-2,4,6-trioxa-11,15-diaza-3lambda~5~,5lambda~5~-diphosphaheptadecan-17-yl} (9z)-octadec-9-enethioate (non-preferred name) (1 kDa, Ligand)
- Tetrameric human 12-lipoxygenase bound to endogenous inhibitor oleoyl-coa (300 kDa, Complex from Homo sapiens)