Electron Microscopy Data Bank

EMD-0338

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MS2 / F-pilus complex

Release date: July 31, 2019
Resolution: 32.3 Å
EM Method: Subtomogram averaging

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

Ms2 / f-pilus complex (Complex from Enterobacterio phage MS2)

EMD-0448

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F-pilus/MS2 complex (Class 2 map with genome masked out)

Release date: July 24, 2019
Resolution: 5.6 Å
EM Method: Single-particle

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

F-plius (Complex from Escherichia coli)
F-pilus/ms2 complex (Complex)
Enterobacteria phage ms2 (Complex from Enterobacteria phage MS2)

EMD-0450

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F-pilus/MS2 complex (Class 3 map with genome masked out)

Release date: July 24, 2019
Resolution: 7.3 Å
EM Method: Single-particle

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

EMD-0451

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Enterobacteria phage MS2

Release date: July 24, 2019
Resolution: 6.7 Å
EM Method: Single-particle

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

Enterobacteria phage ms2 (Virus from Enterobacteria phage MS2)

EMD-0453

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F-pilus/MS2 complex (all particles map with genome masked out)

Release date: July 24, 2019
Resolution: 5.0 Å
EM Method: Single-particle

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

EMD-9397

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F-pilus/MS2 Maturation protein complex

Release date: July 24, 2019
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 6nm5
Q-score: 0.136

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

F-pilus/ms2 maturation protein complex (Complex)
Type iv conjugative transfer system pilin traa (6 kDa, Protein from Escherichia coli)
Enterobacteria phage ms2 maturation protein (Complex from Escherichia coli)
(2r)-2,3-dihydroxypropyl ethyl hydrogen (s)-phosphate (200 Da, Ligand)
Maturation protein (44 kDa, Protein from Enterobacteria phage MS2)
F-pilus (Complex from Enterobacteria phage MS2)

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EMD-9399

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F-pilus/MS2 complex (Class 1 map with genome masked out)

Release date: July 24, 2019
Resolution: 5.8 Å
EM Method: Single-particle

Meng R, Jiang M, Cui Z, Chang JY, Yang K, Jakana J, Yu X, Wang Z, Hu B, Zhang J

Nat Commun (2019) 10 pp. 3130-3130 [ Pubmed: 31311931 DOI: doi:10.1038/s41467-019-11126-8 ]

Sample:

EMD-0531

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AcrAB subcomplex - In situ structure and assembly of multidrug efflux pump AcrAB-TolC

Release date: June 26, 2019
Resolution: 16.0 Å
EM Method: Subtomogram averaging

Shi X, Chen M, Yu Z, Bell JM, Wang H, Forrester I, Villarreal H, Jakana J, Du D, Luisi BF, Ludtke SJ, Wang Z

Nat Commun (2019) 10 pp. 2635-2635 [ Pubmed: 31201302 DOI: doi:10.1038/s41467-019-10512-6 ]

Sample:

E.coli cell overexpressing acrab-tolc (Cell from Escherichia coli BL21(DE3))

EMD-0532

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AcrAB-TolC close state - In situ structure and assembly of multidrug efflux pump AcrAB-TolC

Release date: June 26, 2019
Resolution: 14.5 Å
EM Method: Subtomogram averaging

Shi X, Chen M, Yu Z, Bell JM, Wang H, Forrester I, Villarreal H, Jakana J, Du D, Luisi BF, Ludtke SJ, Wang Z

Nat Commun (2019) 10 pp. 2635-2635 [ Pubmed: 31201302 DOI: doi:10.1038/s41467-019-10512-6 ]

Sample:

E.coli cell overexpressing acrab-tolc (Cell from Escherichia coli BL21(DE3))

EMD-0533

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AcrAB-TolC open state - In situ structure and assembly of multidrug efflux pump AcrAB-TolC

Release date: June 26, 2019
Resolution: 20.0 Å
EM Method: Subtomogram averaging

Shi X, Chen M, Yu Z, Bell JM, Wang H, Forrester I, Villarreal H, Jakana J, Du D, Luisi BF, Ludtke SJ, Wang Z

Nat Commun (2019) 10 pp. 2635-2635 [ Pubmed: 31201302 DOI: doi:10.1038/s41467-019-10512-6 ]

Sample:

E.coli cell overexpressing acrab-tolc treated with acrb inhibitor (Cell from Escherichia coli BL21(DE3))

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